Fig.1 Experimental design showing treatments and husbandry events during the course of the study. Symbols indicate when an experimental event occurred at each time point. (1) 260 fish were assigned and acclimated to one of three water temperature groups (e.g., 28°C, 32°C, or 35°C) and reared from 0 to 164 days post fertilization (dpf). (2a) At 164 dpf (or 0 days post exposure; DPE), fecal collections were collected from a random selection of five fish per tank (n = 60). Additionally, histological and wet mount assessments were conducted on selected fish to assess presence of infection and infection burden. (1b) Afterwards, a cohort of fish from each water temperature group were exposed to Pseudocapillaria tomentosa. (3-6) Subsequent fecal samples were collected and histopathological assessments were conducted at 14 dpe (n = 54), (3) 21 dpe (n = 48), (4) 28 dpe (n = 47), and (5) 42 dpe (n = 51).
Fig. 2 Effects of water temperature on gut microbiomes of parasite unexposed zebrafish. (A) Simpson’s Index of diversity shows that gut microbiome diversity significantly differs between fish reared at 28°C and 35°C water temperatures. (B) Capscale ordination based on the Bray-Curtis dissimilarity of gut microbiome composition. The analysis shows that gut microbiome composition significantly differs between fish reared at different water temperatures. (C) Simpson’s Index of diversity shows microbial gut diversity increases with time from 0 days post exposure (dpe) to 42 dpe, irrespective of water temperature. (D) Capscale ordination of gut microbiome composition based on the Bray-Curtis dissimilarity constrained on the main effects of water temperature and time, and their interaction. The analysis shows that shows that gut microbiome composition differs between fish across time depending on water temperature. Ribbons and ellipses indicate 95% confidence interval. Only statistically significant relationships are shown. A “*” indicates statistical significance below the “0.05” level. Black arrows indicate direction of greatest change in the indicated covariates.
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| GLM Results | |||||
| glm(Alpha.Score ~ Temperature); Unexposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| Shannon | |||||
| (Intercept) | −0.754 | 0.091 | −8.309 | <0.001 | **** |
| Temperature32 | 0.051 | 0.127 | 0.400 | ≥0.25 | ns |
| Temperature35 | 0.054 | 0.129 | 0.421 | ≥0.25 | ns |
| Simpson | |||||
| (Intercept) | −0.101 | 0.092 | −1.103 | ≥0.25 | ns |
| Temperature32 | 0.132 | 0.128 | 1.030 | ≥0.25 | ns |
| Temperature35 | 0.340 | 0.131 | 2.591 | 0.010 | * |
| Richness | |||||
| (Intercept) | −0.231 | 0.075 | −3.079 | 0.002 | ** |
| Temperature32 | 0.047 | 0.105 | 0.446 | ≥0.25 | ns |
| Temperature35 | −0.473 | 0.110 | −4.299 | <0.001 | **** |
| Phylogenetic | |||||
| (Intercept) | −0.255 | 0.072 | −3.540 | <0.001 | *** |
| Temperature32 | 0.068 | 0.101 | 0.673 | ≥0.25 | ns |
| Temperature35 | −0.475 | 0.106 | −4.482 | <0.001 | **** |
| ANOVA of GLM | ||||
| ANOVA(GLM(Alpha.Score ~ Temperature), type = 2); Unexposed fish | ||||
| term | statistic | df | p.value | sig |
|---|---|---|---|---|
| Shannon | ||||
| Temperature | 0.225 | 2.000 | ≥0.25 | ns |
| Simpson | ||||
| Temperature | 6.830 | 2.000 | 0.033 | * |
| Richness | ||||
| Temperature | 27.512 | 2.000 | <0.001 | **** |
| Phylogenetic | ||||
| Temperature | 31.449 | 2.000 | <0.001 | **** |
| Pairwise Tukey's HSD, p.adj: Dunnett | ||||||||
| Tukey(Alpha.Score ~ Temperature); Unexposed fish | ||||||||
| term | .y. | group1 | group2 | estimate | std.error | statistic | adj.p.value | Variable |
|---|---|---|---|---|---|---|---|---|
| Shannon | ||||||||
| Temperature | Alpha.Score | 32 | 28 | 0.051 | 0.127 | 0.400 | ≥0.25 | Temperature |
| Temperature | Alpha.Score | 35 | 28 | 0.054 | 0.129 | 0.421 | ≥0.25 | Temperature |
| Temperature | Alpha.Score | 35 | 32 | 0.004 | 0.127 | 0.029 | ≥0.25 | Temperature |
| Simpson | ||||||||
| Temperature | Alpha.Score | 32 | 28 | 0.132 | 0.128 | 1.030 | ≥0.25 | Temperature |
| Temperature | Alpha.Score | 35 | 28 | 0.340 | 0.131 | 2.591 | 0.026 | Temperature |
| Temperature | Alpha.Score | 35 | 32 | 0.207 | 0.130 | 1.596 | 0.247 | Temperature |
| Richness | ||||||||
| Temperature | Alpha.Score | 32 | 28 | 0.047 | 0.105 | 0.446 | ≥0.25 | Temperature |
| Temperature | Alpha.Score | 35 | 28 | −0.473 | 0.110 | −4.299 | <0.001 | Temperature |
| Temperature | Alpha.Score | 35 | 32 | −0.520 | 0.109 | −4.768 | <0.001 | Temperature |
| Phylogenetic | ||||||||
| Temperature | Alpha.Score | 32 | 28 | 0.068 | 0.101 | 0.673 | ≥0.25 | Temperature |
| Temperature | Alpha.Score | 35 | 28 | −0.475 | 0.106 | −4.482 | <0.001 | Temperature |
| Temperature | Alpha.Score | 35 | 32 | −0.543 | 0.105 | −5.172 | <0.001 | Temperature |
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| ADONIS2 | ||||||
| adonis2(Beta Distance ~ Temperature); Unexposed fish | ||||||
| term | df | SumOfSqs | R2 | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 2.294 | 0.133 | 12.900 | 0.001 | *** |
| Residual | 168.000 | 14.936 | 0.867 | NA | NA | NA |
| Total | 170.000 | 17.229 | 1.000 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 3.862 | 0.099 | 9.178 | 0.001 | *** |
| Residual | 168.000 | 35.344 | 0.901 | NA | NA | NA |
| Total | 170.000 | 39.206 | 1.000 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 1.422 | 0.102 | 9.566 | 0.001 | *** |
| Residual | 168.000 | 12.485 | 0.898 | NA | NA | NA |
| Total | 170.000 | 13.907 | 1.000 | NA | NA | NA |
| ANOVA: Homogeneity of Dispersion | ||||||
| ANOVA(Beta Disperson ~ Temperature); Unexposed fish | ||||||
| term | df | sumsq | meansq | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 0.008 | 0.004 | 0.386 | ≥0.25 | ns |
| Residual | 168.000 | 1.771 | 0.011 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 0.008 | 0.004 | 0.873 | ≥0.25 | ns |
| Residual | 168.000 | 0.759 | 0.005 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 0.005 | 0.003 | 0.369 | ≥0.25 | ns |
| Residual | 168.000 | 1.143 | 0.007 | NA | NA | NA |
| Tukey: Homogeneity of Dispersion | ||||||||
| Tukey(Beta Disperson ~ Temperature); Unexposed fish | ||||||||
| .y. | term | group1 | group2 | estimate | conf.low | conf.high | adj.p.value | sig |
|---|---|---|---|---|---|---|---|---|
| bray | ||||||||
| Distance | Temperature | 32 | 28 | −0.011 | −0.056 | 0.034 | ≥0.25 | ns |
| Distance | Temperature | 35 | 28 | 0.005 | −0.041 | 0.051 | ≥0.25 | ns |
| Distance | Temperature | 35 | 32 | 0.016 | −0.029 | 0.062 | ≥0.25 | ns |
| canberra | ||||||||
| Distance | Temperature | 32 | 28 | −0.001 | −0.031 | 0.028 | ≥0.25 | ns |
| Distance | Temperature | 35 | 28 | 0.014 | −0.016 | 0.044 | ≥0.25 | ns |
| Distance | Temperature | 35 | 32 | 0.015 | −0.015 | 0.045 | ≥0.25 | ns |
| gunifrac | ||||||||
| Distance | Temperature | 32 | 28 | −0.013 | −0.049 | 0.023 | ≥0.25 | ns |
| Distance | Temperature | 35 | 28 | −0.004 | −0.040 | 0.033 | ≥0.25 | ns |
| Distance | Temperature | 35 | 32 | 0.009 | −0.027 | 0.046 | ≥0.25 | ns |
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| GLM Results | |||||
| glm(Alpha.Score ~ Temperature*DPE); Unexposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| Shannon | |||||
| (Intercept) | −1.074 | 0.138 | −7.754 | <0.001 | **** |
| Temperature32 | 0.075 | 0.193 | 0.386 | ≥0.25 | ns |
| Temperature35 | 0.154 | 0.193 | 0.797 | ≥0.25 | ns |
| DPE | 0.018 | 0.006 | 3.086 | 0.002 | ** |
| Temperature32:DPE | −0.001 | 0.008 | −0.170 | ≥0.25 | ns |
| Temperature35:DPE | −0.005 | 0.008 | −0.585 | ≥0.25 | ns |
| Simpson | |||||
| (Intercept) | −0.433 | 0.134 | −3.245 | 0.001 | ** |
| Temperature32 | 0.157 | 0.186 | 0.841 | ≥0.25 | ns |
| Temperature35 | 0.408 | 0.187 | 2.177 | 0.031 | * |
| DPE | 0.019 | 0.006 | 3.293 | 0.001 | ** |
| Temperature32:DPE | −0.001 | 0.008 | −0.173 | ≥0.25 | ns |
| Temperature35:DPE | −0.003 | 0.008 | −0.343 | ≥0.25 | ns |
| Richness | |||||
| (Intercept) | −0.651 | 0.108 | −6.017 | <0.001 | **** |
| Temperature32 | 0.189 | 0.150 | 1.257 | 0.211 | ns |
| Temperature35 | −0.059 | 0.154 | −0.383 | ≥0.25 | ns |
| DPE | 0.024 | 0.005 | 5.137 | <0.001 | **** |
| Temperature32:DPE | −0.008 | 0.006 | −1.251 | 0.213 | ns |
| Temperature35:DPE | −0.024 | 0.007 | −3.476 | <0.001 | *** |
| Phylogenetic | |||||
| (Intercept) | −0.709 | 0.102 | −6.970 | <0.001 | **** |
| Temperature32 | 0.237 | 0.141 | 1.682 | 0.095 | ns |
| Temperature35 | 0.010 | 0.144 | 0.072 | ≥0.25 | ns |
| DPE | 0.026 | 0.004 | 5.907 | <0.001 | **** |
| Temperature32:DPE | −0.010 | 0.006 | −1.579 | 0.116 | ns |
| Temperature35:DPE | −0.028 | 0.006 | −4.344 | <0.001 | **** |
| ANOVA of GLM | ||||
| ANOVA(GLM(Alpha.Score ~ Temperature*Time), type = 2); Unexposed fish | ||||
| term | statistic | df | p.value | sig |
|---|---|---|---|---|
| Shannon | ||||
| Temperature | 0.322 | 2.000 | ≥0.25 | ns |
| DPE | 22.895 | 1.000 | <0.001 | **** |
| Temperature:DPE | 0.363 | 2.000 | ≥0.25 | ns |
| Simpson | ||||
| Temperature | 8.551 | 2.000 | 0.014 | * |
| DPE | 28.161 | 1.000 | <0.001 | **** |
| Temperature:DPE | 0.118 | 2.000 | ≥0.25 | ns |
| Richness | ||||
| Temperature | 31.759 | 2.000 | <0.001 | **** |
| DPE | 27.201 | 1.000 | <0.001 | **** |
| Temperature:DPE | 12.460 | 2.000 | 0.002 | ** |
| Phylogenetic | ||||
| Temperature | 38.358 | 2.000 | <0.001 | **** |
| DPE | 31.813 | 1.000 | <0.001 | **** |
| Temperature:DPE | 19.500 | 2.000 | <0.001 | **** |
| Pairwise Tukey's HSD, p.adj: Dunnett | ||||||||||
| Tukey(Alpha.Score ~ Temperature*Time); Unexposed fish | ||||||||||
| Temperature | .y. | term | group1 | group2 | estimate | std.error | statistic | adj.p.value | Variable | Group |
|---|---|---|---|---|---|---|---|---|---|---|
| Shannon | ||||||||||
| 28 | Alpha.Score | DPE | 14 | 0 | 1.065 | 0.294 | 3.625 | 0.003 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 0 | 0.722 | 0.281 | 2.566 | 0.076 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 0 | 1.187 | 0.274 | 4.335 | <0.001 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 0 | 0.548 | 0.296 | 1.853 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 14 | −0.343 | 0.317 | −1.083 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 14 | 0.122 | 0.310 | 0.392 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 14 | −0.517 | 0.330 | −1.567 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 21 | 0.465 | 0.299 | 1.558 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 21 | −0.174 | 0.319 | −0.545 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 28 | −0.639 | 0.312 | −2.045 | 0.243 | DPE | 28 |
| 32 | Alpha.Score | DPE | 14 | 0 | 0.006 | 0.250 | 0.022 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 0 | 0.112 | 0.247 | 0.452 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 0 | 0.420 | 0.248 | 1.695 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 0 | 0.694 | 0.236 | 2.943 | 0.027 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 14 | 0.106 | 0.286 | 0.371 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 14 | 0.414 | 0.287 | 1.445 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 14 | 0.688 | 0.276 | 2.490 | 0.092 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 21 | 0.308 | 0.283 | 1.087 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 21 | 0.582 | 0.273 | 2.131 | 0.205 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 28 | 0.274 | 0.274 | 1.001 | ≥0.25 | DPE | 32 |
| 35 | Alpha.Score | DPE | 14 | 0 | −0.006 | 0.210 | −0.027 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 0 | −0.249 | 0.218 | −1.145 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 0 | 0.232 | 0.204 | 1.138 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 0 | 0.661 | 0.207 | 3.194 | 0.012 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 14 | −0.243 | 0.253 | −0.962 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 14 | 0.238 | 0.242 | 0.985 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 14 | 0.667 | 0.244 | 2.733 | 0.049 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 21 | 0.481 | 0.248 | 1.938 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 21 | 0.910 | 0.251 | 3.628 | 0.003 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 28 | 0.429 | 0.239 | 1.792 | ≥0.25 | DPE | 35 |
| Simpson | ||||||||||
| 28 | Alpha.Score | DPE | 14 | 0 | 1.256 | 0.273 | 4.611 | <0.001 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 0 | 0.761 | 0.247 | 3.076 | 0.018 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 0 | 1.334 | 0.254 | 5.241 | <0.001 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 0 | 0.514 | 0.257 | 2.002 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 14 | −0.496 | 0.302 | −1.644 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 14 | 0.077 | 0.308 | 0.251 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 14 | −0.742 | 0.310 | −2.398 | 0.114 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 21 | 0.573 | 0.285 | 2.008 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 21 | −0.246 | 0.288 | −0.857 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 28 | −0.819 | 0.294 | −2.789 | 0.042 | DPE | 28 |
| 32 | Alpha.Score | DPE | 14 | 0 | 0.065 | 0.249 | 0.261 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 0 | 0.142 | 0.249 | 0.569 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 0 | 0.592 | 0.261 | 2.272 | 0.152 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 0 | 0.724 | 0.254 | 2.850 | 0.035 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 14 | 0.077 | 0.287 | 0.267 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 14 | 0.527 | 0.297 | 1.773 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 14 | 0.659 | 0.292 | 2.260 | 0.156 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 21 | 0.451 | 0.297 | 1.517 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 21 | 0.582 | 0.291 | 1.999 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 28 | 0.132 | 0.302 | 0.436 | ≥0.25 | DPE | 32 |
| 35 | Alpha.Score | DPE | 14 | 0 | 0.149 | 0.219 | 0.683 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 0 | −0.167 | 0.218 | −0.766 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 0 | 0.413 | 0.222 | 1.858 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 0 | 0.836 | 0.244 | 3.431 | 0.005 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 14 | −0.316 | 0.257 | −1.232 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 14 | 0.263 | 0.260 | 1.013 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 14 | 0.687 | 0.279 | 2.465 | 0.097 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 21 | 0.579 | 0.260 | 2.233 | 0.165 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 21 | 1.003 | 0.278 | 3.604 | 0.003 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 28 | 0.424 | 0.282 | 1.506 | ≥0.25 | DPE | 35 |
| Richness | ||||||||||
| 28 | Alpha.Score | DPE | 14 | 0 | 0.432 | 0.236 | 1.836 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 0 | 0.550 | 0.218 | 2.526 | 0.084 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 0 | 0.767 | 0.218 | 3.525 | 0.004 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 0 | 0.970 | 0.227 | 4.278 | <0.001 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 14 | 0.118 | 0.263 | 0.449 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 14 | 0.335 | 0.262 | 1.275 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 14 | 0.537 | 0.270 | 1.990 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 21 | 0.217 | 0.247 | 0.880 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 21 | 0.419 | 0.255 | 1.647 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 28 | 0.203 | 0.254 | 0.796 | ≥0.25 | DPE | 28 |
| 32 | Alpha.Score | DPE | 14 | 0 | −0.170 | 0.177 | −0.965 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 0 | −0.026 | 0.175 | −0.147 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 0 | 0.187 | 0.180 | 1.041 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 0 | 0.769 | 0.176 | 4.359 | <0.001 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 14 | 0.145 | 0.204 | 0.711 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 14 | 0.358 | 0.208 | 1.721 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 14 | 0.939 | 0.205 | 4.585 | <0.001 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 21 | 0.213 | 0.206 | 1.031 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 21 | 0.794 | 0.203 | 3.906 | <0.001 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 28 | 0.581 | 0.208 | 2.801 | 0.040 | DPE | 32 |
| 35 | Alpha.Score | DPE | 14 | 0 | −0.560 | 0.214 | −2.612 | 0.067 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 0 | −0.551 | 0.214 | −2.578 | 0.073 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 0 | 0.110 | 0.196 | 0.561 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 0 | 0.007 | 0.206 | 0.036 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 14 | 0.008 | 0.259 | 0.032 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 14 | 0.670 | 0.245 | 2.733 | 0.048 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 14 | 0.567 | 0.253 | 2.240 | 0.162 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 21 | 0.661 | 0.245 | 2.702 | 0.053 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 21 | 0.559 | 0.253 | 2.210 | 0.173 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 28 | −0.103 | 0.238 | −0.431 | ≥0.25 | DPE | 35 |
| Phylogenetic | ||||||||||
| 28 | Alpha.Score | DPE | 14 | 0 | 0.452 | 0.229 | 1.969 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 0 | 0.679 | 0.211 | 3.210 | 0.011 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 0 | 0.872 | 0.212 | 4.120 | <0.001 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 0 | 1.012 | 0.220 | 4.602 | <0.001 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 14 | 0.227 | 0.254 | 0.893 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 14 | 0.420 | 0.254 | 1.651 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 14 | 0.560 | 0.261 | 2.142 | 0.200 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 21 | 0.193 | 0.238 | 0.809 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 21 | 0.333 | 0.246 | 1.353 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 28 | 0.140 | 0.246 | 0.569 | ≥0.25 | DPE | 28 |
| 32 | Alpha.Score | DPE | 14 | 0 | −0.030 | 0.164 | −0.181 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 0 | 0.154 | 0.163 | 0.945 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 0 | 0.260 | 0.168 | 1.550 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 0 | 0.753 | 0.164 | 4.594 | <0.001 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 14 | 0.183 | 0.188 | 0.974 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 14 | 0.290 | 0.193 | 1.503 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 14 | 0.783 | 0.190 | 4.130 | <0.001 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 21 | 0.107 | 0.192 | 0.556 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 21 | 0.599 | 0.188 | 3.185 | 0.013 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 28 | 0.493 | 0.193 | 2.555 | 0.078 | DPE | 32 |
| 35 | Alpha.Score | DPE | 14 | 0 | −0.393 | 0.204 | −1.929 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 0 | −0.453 | 0.206 | −2.198 | 0.177 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 0 | 0.090 | 0.192 | 0.468 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 0 | −0.111 | 0.204 | −0.542 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 14 | −0.059 | 0.246 | −0.241 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 14 | 0.483 | 0.235 | 2.057 | 0.235 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 14 | 0.282 | 0.245 | 1.153 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 21 | 0.542 | 0.237 | 2.292 | 0.145 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 21 | 0.342 | 0.247 | 1.386 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 28 | −0.200 | 0.235 | −0.853 | ≥0.25 | DPE | 35 |
Click on tabs to display tables. Scroll to see additional rows.
| ADONIS2 | ||||||
| adonis2(Beta Distance ~ Temperature*DPE); Unexposed fish | ||||||
| term | df | SumOfSqs | R2 | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 2.294 | 0.133 | 16.443 | 0.001 | *** |
| DPE | 1.000 | 2.531 | 0.147 | 36.284 | 0.001 | *** |
| Temperature:DPE | 2.000 | 0.896 | 0.052 | 6.425 | 0.001 | *** |
| Residual | 165.000 | 11.509 | 0.668 | NA | NA | NA |
| Total | 170.000 | 17.229 | 1.000 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 3.862 | 0.099 | 10.667 | 0.001 | *** |
| DPE | 1.000 | 3.655 | 0.093 | 20.192 | 0.001 | *** |
| Temperature:DPE | 2.000 | 1.820 | 0.046 | 5.026 | 0.001 | *** |
| Residual | 165.000 | 29.869 | 0.762 | NA | NA | NA |
| Total | 170.000 | 39.206 | 1.000 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 1.422 | 0.102 | 11.984 | 0.001 | *** |
| DPE | 1.000 | 2.123 | 0.153 | 35.785 | 0.001 | *** |
| Temperature:DPE | 2.000 | 0.574 | 0.041 | 4.840 | 0.001 | *** |
| Residual | 165.000 | 9.788 | 0.704 | NA | NA | NA |
| Total | 170.000 | 13.907 | 1.000 | NA | NA | NA |
| ANOVA: Homogeneity of Dispersion | ||||||
| ANOVA(Beta Disperson ~ Temperature); Unexposed fish | ||||||
| term | df | sumsq | meansq | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temp.DPE | 14.000 | 0.290 | 0.021 | 2.348 | 0.006 | ** |
| Residual | 156.000 | 1.376 | 0.009 | NA | NA | NA |
| canberra | ||||||
| Temp.DPE | 14.000 | 0.250 | 0.018 | 3.344 | <0.001 | *** |
| Residual | 156.000 | 0.831 | 0.005 | NA | NA | NA |
| gunifrac | ||||||
| Temp.DPE | 14.000 | 0.249 | 0.018 | 2.986 | <0.001 | *** |
| Residual | 156.000 | 0.929 | 0.006 | NA | NA | NA |
| Tukey: Homogeneity of Dispersion | ||||||||
| Tukey(Beta Disperson ~ Temperature*DPE); Unexposed fish | ||||||||
| .y. | term | group1 | group2 | estimate | conf.low | conf.high | adj.p.value | sig |
|---|---|---|---|---|---|---|---|---|
| bray | ||||||||
| Distance | Temp.DPE | 28°C__14DPE | 28°C__0DPE | 0.024 | −0.112 | 0.159 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__21DPE | 28°C__0DPE | 0.052 | −0.074 | 0.177 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__0DPE | 0.007 | −0.118 | 0.133 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__0DPE | −0.017 | −0.147 | 0.113 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__0DPE | 0.097 | −0.005 | 0.199 | 0.085 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__0DPE | 0.041 | −0.085 | 0.166 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__0DPE | −0.018 | −0.143 | 0.108 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__0DPE | 0.028 | −0.102 | 0.158 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__0DPE | 0.017 | −0.108 | 0.142 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__0DPE | 0.044 | −0.058 | 0.146 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__0DPE | 0.004 | −0.126 | 0.134 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__0DPE | −0.004 | −0.134 | 0.126 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__0DPE | 0.033 | −0.097 | 0.162 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__0DPE | 0.146 | 0.011 | 0.282 | 0.021 | * |
| Distance | Temp.DPE | 28°C__21DPE | 28°C__14DPE | 0.028 | −0.126 | 0.181 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__14DPE | −0.016 | −0.170 | 0.137 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__14DPE | −0.040 | −0.198 | 0.117 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__14DPE | 0.073 | −0.062 | 0.209 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__14DPE | 0.017 | −0.136 | 0.171 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__14DPE | −0.041 | −0.195 | 0.112 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__14DPE | 0.005 | −0.153 | 0.162 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__14DPE | −0.006 | −0.160 | 0.147 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__14DPE | 0.020 | −0.115 | 0.156 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__14DPE | −0.019 | −0.176 | 0.138 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__14DPE | −0.028 | −0.185 | 0.129 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__14DPE | 0.009 | −0.148 | 0.166 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__14DPE | 0.123 | −0.039 | 0.284 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__21DPE | −0.044 | −0.189 | 0.101 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__21DPE | −0.068 | −0.217 | 0.081 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__21DPE | 0.045 | −0.080 | 0.171 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__21DPE | −0.011 | −0.156 | 0.134 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__21DPE | −0.069 | −0.214 | 0.076 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__21DPE | −0.023 | −0.172 | 0.125 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__21DPE | −0.034 | −0.179 | 0.110 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__21DPE | −0.008 | −0.133 | 0.118 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__21DPE | −0.047 | −0.196 | 0.102 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__21DPE | −0.056 | −0.205 | 0.093 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__21DPE | −0.019 | −0.168 | 0.130 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__21DPE | 0.095 | −0.059 | 0.248 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__28DPE | −0.024 | −0.173 | 0.125 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__28DPE | 0.089 | −0.036 | 0.215 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__28DPE | 0.033 | −0.111 | 0.178 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__28DPE | −0.025 | −0.170 | 0.120 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__28DPE | 0.021 | −0.128 | 0.169 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__28DPE | 0.010 | −0.135 | 0.154 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__28DPE | 0.036 | −0.089 | 0.162 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__28DPE | −0.003 | −0.152 | 0.146 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__28DPE | −0.012 | −0.161 | 0.137 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__28DPE | 0.025 | −0.124 | 0.174 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__28DPE | 0.139 | −0.015 | 0.292 | 0.125 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__42DPE | 0.114 | −0.016 | 0.243 | 0.161 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__42DPE | 0.057 | −0.091 | 0.206 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__42DPE | −0.001 | −0.150 | 0.148 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__42DPE | 0.045 | −0.108 | 0.197 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__42DPE | 0.034 | −0.115 | 0.183 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__42DPE | 0.061 | −0.069 | 0.191 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__42DPE | 0.021 | −0.131 | 0.174 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__42DPE | 0.012 | −0.140 | 0.165 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__42DPE | 0.049 | −0.103 | 0.202 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__42DPE | 0.163 | 0.006 | 0.320 | 0.034 | * |
| Distance | Temp.DPE | 32°C__14DPE | 32°C__0DPE | −0.056 | −0.182 | 0.069 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__0DPE | −0.114 | −0.240 | 0.011 | 0.115 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__0DPE | −0.069 | −0.199 | 0.061 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__0DPE | −0.080 | −0.205 | 0.046 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__0DPE | −0.053 | −0.155 | 0.049 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__0DPE | −0.092 | −0.222 | 0.037 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__0DPE | −0.101 | −0.231 | 0.029 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__0DPE | −0.064 | −0.194 | 0.066 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__0DPE | 0.049 | −0.086 | 0.185 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__14DPE | −0.058 | −0.203 | 0.086 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__14DPE | −0.013 | −0.161 | 0.136 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__14DPE | −0.024 | −0.168 | 0.121 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__14DPE | 0.003 | −0.122 | 0.129 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__14DPE | −0.036 | −0.185 | 0.112 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__14DPE | −0.045 | −0.194 | 0.104 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__14DPE | −0.008 | −0.157 | 0.141 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__14DPE | 0.105 | −0.048 | 0.259 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__21DPE | 0.046 | −0.103 | 0.194 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__21DPE | 0.035 | −0.110 | 0.179 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__21DPE | 0.061 | −0.064 | 0.187 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__21DPE | 0.022 | −0.127 | 0.171 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__21DPE | 0.013 | −0.136 | 0.162 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__21DPE | 0.050 | −0.099 | 0.199 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__21DPE | 0.164 | 0.010 | 0.317 | 0.025 | * |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__28DPE | −0.011 | −0.160 | 0.138 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__28DPE | 0.016 | −0.114 | 0.146 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__28DPE | −0.024 | −0.176 | 0.129 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__28DPE | −0.033 | −0.185 | 0.120 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__28DPE | 0.004 | −0.148 | 0.157 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__28DPE | 0.118 | −0.039 | 0.275 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__42DPE | 0.027 | −0.099 | 0.152 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__42DPE | −0.013 | −0.161 | 0.136 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__42DPE | −0.021 | −0.170 | 0.127 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__42DPE | 0.015 | −0.133 | 0.164 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__42DPE | 0.129 | −0.024 | 0.283 | 0.209 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 35°C__0DPE | −0.039 | −0.169 | 0.090 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__0DPE | −0.048 | −0.178 | 0.082 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__0DPE | −0.011 | −0.141 | 0.119 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__0DPE | 0.102 | −0.033 | 0.238 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__14DPE | −0.009 | −0.161 | 0.144 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__14DPE | 0.028 | −0.124 | 0.181 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__14DPE | 0.142 | −0.016 | 0.299 | 0.127 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__21DPE | 0.037 | −0.116 | 0.190 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__21DPE | 0.151 | −0.007 | 0.308 | 0.076 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__28DPE | 0.114 | −0.044 | 0.271 | ≥0.25 | ns |
| canberra | ||||||||
| Distance | Temp.DPE | 28°C__14DPE | 28°C__0DPE | −0.033 | −0.138 | 0.072 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__21DPE | 28°C__0DPE | −0.005 | −0.103 | 0.092 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__0DPE | −0.074 | −0.171 | 0.024 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__0DPE | −0.069 | −0.170 | 0.032 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__0DPE | 0.035 | −0.045 | 0.115 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__0DPE | −0.021 | −0.119 | 0.076 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__0DPE | −0.070 | −0.167 | 0.028 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__0DPE | −0.056 | −0.157 | 0.045 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__0DPE | −0.068 | −0.165 | 0.030 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__0DPE | 0.008 | −0.071 | 0.088 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__0DPE | −0.041 | −0.142 | 0.060 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__0DPE | −0.047 | −0.148 | 0.054 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__0DPE | −0.052 | −0.153 | 0.049 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__0DPE | 0.045 | −0.061 | 0.150 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__21DPE | 28°C__14DPE | 0.028 | −0.091 | 0.147 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__14DPE | −0.040 | −0.160 | 0.079 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__14DPE | −0.035 | −0.158 | 0.087 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__14DPE | 0.068 | −0.037 | 0.174 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__14DPE | 0.012 | −0.107 | 0.131 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__14DPE | −0.037 | −0.156 | 0.083 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__14DPE | −0.023 | −0.145 | 0.099 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__14DPE | −0.034 | −0.154 | 0.085 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__14DPE | 0.042 | −0.064 | 0.147 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__14DPE | −0.008 | −0.130 | 0.115 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__14DPE | −0.013 | −0.136 | 0.109 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__14DPE | −0.019 | −0.141 | 0.103 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__14DPE | 0.078 | −0.048 | 0.204 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__21DPE | −0.068 | −0.181 | 0.044 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__21DPE | −0.063 | −0.179 | 0.052 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__21DPE | 0.040 | −0.057 | 0.138 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__21DPE | −0.016 | −0.128 | 0.097 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__21DPE | −0.065 | −0.177 | 0.048 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__21DPE | −0.051 | −0.167 | 0.065 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__21DPE | −0.062 | −0.175 | 0.050 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__21DPE | 0.014 | −0.084 | 0.111 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__21DPE | −0.036 | −0.151 | 0.080 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__21DPE | −0.041 | −0.157 | 0.074 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__21DPE | −0.047 | −0.162 | 0.069 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__21DPE | 0.050 | −0.070 | 0.169 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__28DPE | 0.005 | −0.110 | 0.121 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__28DPE | 0.109 | 0.011 | 0.206 | 0.014 | * |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__28DPE | 0.052 | −0.060 | 0.165 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__28DPE | 0.004 | −0.109 | 0.116 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__28DPE | 0.017 | −0.098 | 0.133 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__28DPE | 0.006 | −0.106 | 0.119 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__28DPE | 0.082 | −0.015 | 0.180 | 0.206 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__28DPE | 0.033 | −0.083 | 0.148 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__28DPE | 0.027 | −0.088 | 0.143 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__28DPE | 0.021 | −0.094 | 0.137 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__28DPE | 0.118 | −0.001 | 0.237 | 0.055 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__42DPE | 0.104 | 0.003 | 0.205 | 0.038 | * |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__42DPE | 0.047 | −0.068 | 0.163 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__42DPE | −0.001 | −0.117 | 0.114 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__42DPE | 0.012 | −0.106 | 0.131 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__42DPE | 0.001 | −0.115 | 0.117 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__42DPE | 0.077 | −0.024 | 0.178 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__42DPE | 0.028 | −0.091 | 0.146 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__42DPE | 0.022 | −0.097 | 0.141 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__42DPE | 0.016 | −0.102 | 0.135 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__42DPE | 0.113 | −0.009 | 0.235 | 0.104 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 32°C__0DPE | −0.056 | −0.154 | 0.041 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__0DPE | −0.105 | −0.202 | −0.007 | 0.022 | * |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__0DPE | −0.091 | −0.192 | 0.010 | 0.125 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__0DPE | −0.103 | −0.200 | −0.005 | 0.029 | * |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__0DPE | −0.027 | −0.106 | 0.053 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__0DPE | −0.076 | −0.177 | 0.025 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__0DPE | −0.082 | −0.183 | 0.019 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__0DPE | −0.087 | −0.188 | 0.014 | 0.175 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__0DPE | 0.009 | −0.096 | 0.115 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__14DPE | −0.049 | −0.161 | 0.064 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__14DPE | −0.035 | −0.151 | 0.081 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__14DPE | −0.046 | −0.159 | 0.066 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__14DPE | 0.030 | −0.068 | 0.127 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__14DPE | −0.020 | −0.135 | 0.096 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__14DPE | −0.025 | −0.141 | 0.090 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__14DPE | −0.031 | −0.147 | 0.085 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__14DPE | 0.066 | −0.054 | 0.185 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__21DPE | 0.014 | −0.102 | 0.129 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__21DPE | 0.002 | −0.110 | 0.115 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__21DPE | 0.078 | −0.019 | 0.176 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__21DPE | 0.029 | −0.087 | 0.145 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__21DPE | 0.023 | −0.092 | 0.139 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__21DPE | 0.018 | −0.098 | 0.133 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__21DPE | 0.114 | −0.005 | 0.234 | 0.076 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__28DPE | −0.011 | −0.127 | 0.104 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__28DPE | 0.065 | −0.036 | 0.166 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__28DPE | 0.015 | −0.103 | 0.134 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__28DPE | 0.010 | −0.109 | 0.128 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__28DPE | 0.004 | −0.115 | 0.123 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__28DPE | 0.101 | −0.022 | 0.223 | 0.237 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__42DPE | 0.076 | −0.021 | 0.173 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__42DPE | 0.027 | −0.089 | 0.142 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__42DPE | 0.021 | −0.095 | 0.137 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__42DPE | 0.015 | −0.100 | 0.131 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__42DPE | 0.112 | −0.007 | 0.231 | 0.091 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 35°C__0DPE | −0.049 | −0.150 | 0.052 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__0DPE | −0.055 | −0.156 | 0.046 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__0DPE | −0.061 | −0.162 | 0.040 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__0DPE | 0.036 | −0.069 | 0.141 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__14DPE | −0.006 | −0.124 | 0.113 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__14DPE | −0.011 | −0.130 | 0.107 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__14DPE | 0.085 | −0.037 | 0.208 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__21DPE | −0.006 | −0.124 | 0.113 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__21DPE | 0.091 | −0.031 | 0.213 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__28DPE | 0.097 | −0.026 | 0.219 | ≥0.25 | ns |
| gunifrac | ||||||||
| Distance | Temp.DPE | 28°C__14DPE | 28°C__0DPE | −0.005 | −0.116 | 0.107 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__21DPE | 28°C__0DPE | 0.038 | −0.065 | 0.141 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__0DPE | −0.010 | −0.113 | 0.093 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__0DPE | −0.033 | −0.140 | 0.073 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__0DPE | 0.067 | −0.017 | 0.151 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__0DPE | 0.018 | −0.085 | 0.121 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__0DPE | −0.023 | −0.126 | 0.080 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__0DPE | −0.014 | −0.121 | 0.093 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__0DPE | −0.026 | −0.129 | 0.077 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__0DPE | 0.029 | −0.055 | 0.113 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__0DPE | −0.015 | −0.122 | 0.092 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__0DPE | −0.020 | −0.127 | 0.087 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__0DPE | −0.065 | −0.171 | 0.042 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__0DPE | 0.091 | −0.020 | 0.202 | 0.245 | ns |
| Distance | Temp.DPE | 28°C__21DPE | 28°C__14DPE | 0.042 | −0.084 | 0.168 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__14DPE | −0.005 | −0.131 | 0.121 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__14DPE | −0.029 | −0.158 | 0.100 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__14DPE | 0.072 | −0.040 | 0.183 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__14DPE | 0.022 | −0.104 | 0.148 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__14DPE | −0.019 | −0.145 | 0.107 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__14DPE | −0.009 | −0.138 | 0.120 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__14DPE | −0.021 | −0.147 | 0.105 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__14DPE | 0.033 | −0.078 | 0.145 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__14DPE | −0.010 | −0.139 | 0.119 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__14DPE | −0.015 | −0.145 | 0.114 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__14DPE | −0.060 | −0.189 | 0.069 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__14DPE | 0.096 | −0.037 | 0.229 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__21DPE | −0.047 | −0.166 | 0.072 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__21DPE | −0.071 | −0.193 | 0.051 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__21DPE | 0.029 | −0.074 | 0.132 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__21DPE | −0.020 | −0.139 | 0.099 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__21DPE | −0.061 | −0.180 | 0.058 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__21DPE | −0.051 | −0.174 | 0.071 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__21DPE | −0.063 | −0.182 | 0.056 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__21DPE | −0.009 | −0.112 | 0.094 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__21DPE | −0.052 | −0.175 | 0.070 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__21DPE | −0.058 | −0.180 | 0.065 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__21DPE | −0.102 | −0.224 | 0.020 | 0.216 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__21DPE | 0.054 | −0.073 | 0.180 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__28DPE | −0.024 | −0.146 | 0.098 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__28DPE | 0.077 | −0.026 | 0.180 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__28DPE | 0.027 | −0.092 | 0.146 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__28DPE | −0.014 | −0.133 | 0.105 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__28DPE | −0.004 | −0.126 | 0.118 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__28DPE | −0.016 | −0.135 | 0.103 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__28DPE | 0.038 | −0.065 | 0.142 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__28DPE | −0.005 | −0.127 | 0.117 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__28DPE | −0.010 | −0.132 | 0.112 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__28DPE | −0.055 | −0.177 | 0.067 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__28DPE | 0.101 | −0.025 | 0.227 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__42DPE | 0.100 | −0.006 | 0.207 | 0.089 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__42DPE | 0.051 | −0.071 | 0.173 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__42DPE | 0.010 | −0.112 | 0.132 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__42DPE | 0.020 | −0.106 | 0.145 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__42DPE | 0.008 | −0.114 | 0.130 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__42DPE | 0.062 | −0.045 | 0.169 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__42DPE | 0.019 | −0.107 | 0.144 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__42DPE | 0.014 | −0.112 | 0.139 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__42DPE | −0.031 | −0.157 | 0.094 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__42DPE | 0.125 | −0.005 | 0.254 | 0.071 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 32°C__0DPE | −0.049 | −0.152 | 0.054 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__0DPE | −0.090 | −0.193 | 0.013 | 0.155 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__0DPE | −0.081 | −0.188 | 0.026 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__0DPE | −0.093 | −0.196 | 0.010 | 0.130 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__0DPE | −0.038 | −0.122 | 0.046 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__0DPE | −0.082 | −0.189 | 0.025 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__0DPE | −0.087 | −0.194 | 0.020 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__0DPE | −0.132 | −0.238 | −0.025 | 0.003 | ** |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__0DPE | 0.024 | −0.087 | 0.135 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__14DPE | −0.041 | −0.160 | 0.078 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__14DPE | −0.031 | −0.154 | 0.091 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__14DPE | −0.043 | −0.162 | 0.076 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__14DPE | 0.011 | −0.092 | 0.114 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__14DPE | −0.032 | −0.155 | 0.090 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__14DPE | −0.037 | −0.160 | 0.085 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__14DPE | −0.082 | −0.204 | 0.040 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__14DPE | 0.074 | −0.053 | 0.200 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__21DPE | 0.010 | −0.113 | 0.132 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__21DPE | −0.002 | −0.121 | 0.117 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__21DPE | 0.052 | −0.051 | 0.155 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__21DPE | 0.009 | −0.114 | 0.131 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__21DPE | 0.004 | −0.119 | 0.126 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__21DPE | −0.041 | −0.163 | 0.081 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__21DPE | 0.115 | −0.011 | 0.241 | 0.119 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__28DPE | −0.012 | −0.134 | 0.110 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__28DPE | 0.043 | −0.064 | 0.149 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__28DPE | −0.001 | −0.126 | 0.124 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__28DPE | −0.006 | −0.132 | 0.119 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__28DPE | −0.051 | −0.176 | 0.075 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__28DPE | 0.105 | −0.024 | 0.234 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__42DPE | 0.054 | −0.049 | 0.157 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__42DPE | 0.011 | −0.111 | 0.133 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__42DPE | 0.006 | −0.117 | 0.128 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__42DPE | −0.039 | −0.161 | 0.083 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__42DPE | 0.117 | −0.009 | 0.243 | 0.102 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 35°C__0DPE | −0.044 | −0.150 | 0.063 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__0DPE | −0.049 | −0.155 | 0.058 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__0DPE | −0.093 | −0.200 | 0.013 | 0.160 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__0DPE | 0.062 | −0.049 | 0.174 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__14DPE | −0.005 | −0.130 | 0.120 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__14DPE | −0.050 | −0.175 | 0.076 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__14DPE | 0.106 | −0.023 | 0.235 | 0.244 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__21DPE | −0.045 | −0.170 | 0.081 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__21DPE | 0.111 | −0.018 | 0.240 | 0.180 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__28DPE | 0.156 | 0.027 | 0.285 | 0.005 | ** |
Click on tabs to reveal supplementary figures and tables.
Fig. 3 Histological sections stained with ### stain in zebrafish exposed to Pseudocapillaria tomentosa examined at ### days post exposure. (A) Nematodes in intestinal lumen. Arrow = ###. (B) Infection outcome analysis of fish exposed to P. tomentosa (n = 89) by temperature. Fish reared at 28°C and 32°C water temperatures had significantly different infection burden to fish reared at 35°C water temperature. Only one fish reared at 35°C was identified as being positively infected by wet mount.
Click on tabs to display tables. Scroll to see additional rows.
| GLM Results | |||||
| glm.nb(Total.Worm.Count ~ Temperature); Exposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| (Intercept) | 1.552 | 0.316 | 4.914 | <0.001 | **** |
| Temperature32 | −0.368 | 0.431 | −0.853 | ≥0.25 | ns |
| Temperature35 | −5.078 | 1.080 | −4.701 | <0.001 | **** |
| ANOVA of GLM | |||
| ANOVA(GLM.NB(Total.Worm.Count ~ Temperature), type = 2); Exposed fish | |||
| term | statistic | df | p.value |
|---|---|---|---|
| Temperature | 55.264 | 2.000 | <0.001 |
| Pairwise Tukey's HSD, p.adj: Dunnett | ||||||||
| Tukey(Total.Worm.Count ~ Temperature); Exposed fish | ||||||||
| term | .y. | group1 | group2 | estimate | std.error | df | statistic | adj.p.value |
|---|---|---|---|---|---|---|---|---|
| Temperature | Total.Worm.Count | 28 | 32 | 0.368 | 0.431 | Inf | 0.853 | ≥0.25 |
| Temperature | Total.Worm.Count | 28 | 35 | 5.078 | 1.080 | Inf | 4.701 | <0.001 |
| Temperature | Total.Worm.Count | 32 | 35 | 4.710 | 1.074 | Inf | 4.386 | <0.001 |
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| GLM Results | |||||
| glm.nb(Total.Worm.Count ~ Temperature*DPE); Exposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| (Intercept) | 0.535 | 0.795 | 0.673 | ≥0.25 | ns |
| Temperature32 | 0.233 | 1.081 | 0.216 | ≥0.25 | ns |
| Temperature35 | −4.474 | 2.964 | −1.509 | 0.131 | ns |
| DPE | 0.035 | 0.027 | 1.304 | 0.192 | ns |
| Temperature32:DPE | −0.019 | 0.038 | −0.501 | ≥0.25 | ns |
| Temperature35:DPE | −0.020 | 0.099 | −0.200 | ≥0.25 | ns |
| ANOVA of GLM | |||
| ANOVA(GLM.NB(Total.Worm.Count ~ Temperature*DPE), type = 2); Exposed fish | |||
| term | statistic | df | p.value |
|---|---|---|---|
| Temperature | 56.284 | 2.000 | <0.001 |
| DPE | 1.790 | 1.000 | 0.181 |
| Temperature:DPE | 0.265 | 2.000 | ≥0.25 |
| Pairwise Tukey's HSD, p.adj: Dunnett | |||||||||
| Tukey(Total.Worm.Count ~ Temperature*DPE); Exposed fish | |||||||||
| DPE | .y. | term | group1 | group2 | estimate | std.error | df | statistic | adj.p.value |
|---|---|---|---|---|---|---|---|---|---|
| 14 | Total.Worm.Count | Temperature | 28 | 32 | −0.229 | 0.279 | Inf | −0.820 | ≥0.25 |
| 14 | Total.Worm.Count | Temperature | 28 | 35 | 38.009 | 22369621.333 | Inf | 0.000 | ≥0.25 |
| 14 | Total.Worm.Count | Temperature | 32 | 35 | 38.238 | 22369621.333 | Inf | 0.000 | ≥0.25 |
| 21 | Total.Worm.Count | Temperature | 28 | 32 | 0.560 | 0.392 | Inf | 1.427 | ≥0.25 |
| 21 | Total.Worm.Count | Temperature | 28 | 35 | 38.222 | 23726566.406 | Inf | 0.000 | ≥0.25 |
| 21 | Total.Worm.Count | Temperature | 32 | 35 | 37.663 | 23726566.406 | Inf | 0.000 | ≥0.25 |
| 28 | Total.Worm.Count | Temperature | 28 | 32 | 0.605 | 0.582 | Inf | 1.040 | ≥0.25 |
| 28 | Total.Worm.Count | Temperature | 28 | 35 | 3.837 | 1.121 | Inf | 3.422 | 0.002 |
| 28 | Total.Worm.Count | Temperature | 32 | 35 | 3.232 | 1.119 | Inf | 2.888 | 0.011 |
| 42 | Total.Worm.Count | Temperature | 28 | 32 | 0.377 | 0.620 | Inf | 0.609 | ≥0.25 |
| 42 | Total.Worm.Count | Temperature | 28 | 35 | 21.230 | 3142.206 | Inf | 0.007 | ≥0.25 |
| 42 | Total.Worm.Count | Temperature | 32 | 35 | 20.853 | 3142.206 | Inf | 0.007 | ≥0.25 |
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| GLM Results | |||||
| glm.nb(Total.Worm.Count ~ Temperature*DPE); Exposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| (Intercept) | 0.535 | 0.795 | 0.673 | ≥0.25 | ns |
| Temperature32 | 0.233 | 1.081 | 0.216 | ≥0.25 | ns |
| Temperature35 | −4.474 | 2.964 | −1.509 | 0.131 | ns |
| DPE | 0.035 | 0.027 | 1.304 | 0.192 | ns |
| Temperature32:DPE | −0.019 | 0.038 | −0.501 | ≥0.25 | ns |
| Temperature35:DPE | −0.020 | 0.099 | −0.200 | ≥0.25 | ns |
| ANOVA of GLM | |||
| ANOVA(GLM.NB(Total.Worm.Count ~ Temperature*DPE), type = 2); Exposed fish | |||
| term | statistic | df | p.value |
|---|---|---|---|
| Temperature | 56.284 | 2.000 | <0.001 |
| DPE | 1.790 | 1.000 | 0.181 |
| Temperature:DPE | 0.265 | 2.000 | ≥0.25 |
Fig. 4 Effects of parasite exposure and water temperature on gut microbiomes of zebrafish. (A) Simpson’s Index of diversity shows that gut microbiome diversity significantly differs between fish reared at 28°C water temperature to fish reared at 32°C and 35°C water temperatures. (B) Capscale ordination based on the Bray-Curtis dissimilarity of gut microbiome composition. The analysis shows that gut microbiome composition significantly differs between parasite exposed fish reared at different water temperatures. (C) Simpson’s Index of diversity shows microbial gut diversity decreases with time from 0 days post exposure (dpe) to 42 dpe in parasite exposed fish reared at 28°C water temperature. (D) Capscale ordination of gut microbiome composition based on the Canberra dissimilarity constrained on the main effects of water temperature and time, and their interaction. The analysis shows that shows that gut microbiome composition differs between parasite exposed fish across time depending on water temperature. Ribbons and ellipses indicate 95% confidence interval. Only statistically significant relationships are shown. A “*” indicates statistical significance below the “0.05” level. Black arrows indicate direction of greatest change in the indicated covariates.
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| GLM Results | |||||
| glm(Alpha.Score ~ Temperature); Exposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| Shannon | |||||
| (Intercept) | −0.936 | 0.233 | −4.021 | <0.001 | *** |
| Temperature32 | 0.909 | 0.301 | 3.017 | 0.003 | ** |
| Temperature35 | 0.516 | 0.296 | 1.741 | 0.085 | ns |
| Simpson | |||||
| (Intercept) | −1.002 | 0.243 | −4.130 | <0.001 | **** |
| Temperature32 | 1.057 | 0.312 | 3.384 | 0.001 | ** |
| Temperature35 | 0.772 | 0.306 | 2.525 | 0.013 | * |
| Richness | |||||
| (Intercept) | −0.681 | 0.190 | −3.583 | <0.001 | *** |
| Temperature32 | 0.703 | 0.251 | 2.800 | 0.006 | ** |
| Temperature35 | 0.242 | 0.247 | 0.978 | ≥0.25 | ns |
| Phylogenetic | |||||
| (Intercept) | −0.681 | 0.180 | −3.789 | <0.001 | *** |
| Temperature32 | 0.648 | 0.237 | 2.730 | 0.008 | ** |
| Temperature35 | 0.161 | 0.235 | 0.688 | ≥0.25 | ns |
| ANOVA of GLM | ||||
| ANOVA(GLM(Alpha.Score ~ Temperature), type = 2); Exposed fish | ||||
| term | statistic | df | p.value | sig |
|---|---|---|---|---|
| Shannon | ||||
| Temperature | 9.440 | 2.000 | 0.009 | ** |
| Simpson | ||||
| Temperature | 12.497 | 2.000 | 0.002 | ** |
| Richness | ||||
| Temperature | 8.575 | 2.000 | 0.014 | * |
| Phylogenetic | ||||
| Temperature | 8.706 | 2.000 | 0.013 | * |
| Pairwise Tukey's HSD, p.adj: Dunnett | ||||||||
| Tukey(Alpha.Score ~ Temperature); Exposed fish | ||||||||
| term | .y. | group1 | group2 | estimate | std.error | statistic | adj.p.value | Variable |
|---|---|---|---|---|---|---|---|---|
| Shannon | ||||||||
| Temperature | Alpha.Score | 32 | 28 | 0.909 | 0.301 | 3.017 | 0.007 | Temperature |
| Temperature | Alpha.Score | 35 | 28 | 0.516 | 0.296 | 1.741 | 0.190 | Temperature |
| Temperature | Alpha.Score | 35 | 32 | −0.393 | 0.265 | −1.482 | ≥0.25 | Temperature |
| Simpson | ||||||||
| Temperature | Alpha.Score | 32 | 28 | 1.057 | 0.312 | 3.384 | 0.002 | Temperature |
| Temperature | Alpha.Score | 35 | 28 | 0.772 | 0.306 | 2.525 | 0.031 | Temperature |
| Temperature | Alpha.Score | 35 | 32 | −0.285 | 0.270 | −1.054 | ≥0.25 | Temperature |
| Richness | ||||||||
| Temperature | Alpha.Score | 32 | 28 | 0.703 | 0.251 | 2.800 | 0.014 | Temperature |
| Temperature | Alpha.Score | 35 | 28 | 0.242 | 0.247 | 0.978 | ≥0.25 | Temperature |
| Temperature | Alpha.Score | 35 | 32 | −0.461 | 0.228 | −2.028 | 0.105 | Temperature |
| Phylogenetic | ||||||||
| Temperature | Alpha.Score | 32 | 28 | 0.648 | 0.237 | 2.730 | 0.017 | Temperature |
| Temperature | Alpha.Score | 35 | 28 | 0.161 | 0.235 | 0.688 | ≥0.25 | Temperature |
| Temperature | Alpha.Score | 35 | 32 | −0.487 | 0.216 | −2.251 | 0.063 | Temperature |
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| ADONIS2 | ||||||
| adonis2(Beta Distance ~ Temperature); Exposed fish | ||||||
| term | df | SumOfSqs | R2 | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 5.218 | 0.251 | 14.447 | 0.001 | *** |
| Residual | 86.000 | 15.530 | 0.749 | NA | NA | NA |
| Total | 88.000 | 20.748 | 1.000 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 3.573 | 0.117 | 5.701 | 0.001 | *** |
| Residual | 86.000 | 26.947 | 0.883 | NA | NA | NA |
| Total | 88.000 | 30.520 | 1.000 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 3.363 | 0.212 | 11.590 | 0.001 | *** |
| Residual | 86.000 | 12.478 | 0.788 | NA | NA | NA |
| Total | 88.000 | 15.841 | 1.000 | NA | NA | NA |
| ANOVA: Homogeneity of Dispersion | ||||||
| ANOVA(Beta Disperson ~ Temperature); Exposed fish | ||||||
| term | df | sumsq | meansq | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 0.364 | 0.182 | 8.708 | <0.001 | *** |
| Residual | 86.000 | 1.796 | 0.021 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 0.314 | 0.157 | 17.419 | <0.001 | **** |
| Residual | 86.000 | 0.774 | 0.009 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 0.310 | 0.155 | 13.567 | <0.001 | **** |
| Residual | 86.000 | 0.983 | 0.011 | NA | NA | NA |
| Tukey: Homogeneity of Dispersion | ||||||||
| Tukey(Beta Disperson ~ Temperature); Exposed fish | ||||||||
| .y. | term | group1 | group2 | estimate | conf.low | conf.high | adj.p.value | sig |
|---|---|---|---|---|---|---|---|---|
| bray | ||||||||
| Distance | Temperature | 32 | 28 | −0.043 | −0.136 | 0.051 | ≥0.25 | ns |
| Distance | Temperature | 35 | 28 | −0.150 | −0.241 | −0.059 | <0.001 | *** |
| Distance | Temperature | 35 | 32 | −0.108 | −0.194 | −0.021 | 0.011 | * |
| canberra | ||||||||
| Distance | Temperature | 32 | 28 | −0.110 | −0.171 | −0.049 | <0.001 | *** |
| Distance | Temperature | 35 | 28 | −0.144 | −0.204 | −0.085 | <0.001 | **** |
| Distance | Temperature | 35 | 32 | −0.034 | −0.091 | 0.022 | ≥0.25 | ns |
| gunifrac | ||||||||
| Distance | Temperature | 32 | 28 | −0.056 | −0.125 | 0.013 | 0.133 | ns |
| Distance | Temperature | 35 | 28 | −0.143 | −0.211 | −0.076 | <0.001 | **** |
| Distance | Temperature | 35 | 32 | −0.087 | −0.151 | −0.023 | 0.005 | ** |
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| GLM Results | |||||
| glm(Alpha.Score ~ Temperature*DPE); Exposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| Shannon | |||||
| (Intercept) | −0.134 | 0.594 | −0.226 | ≥0.25 | ns |
| Temperature32 | 0.228 | 0.774 | 0.295 | ≥0.25 | ns |
| Temperature35 | −0.428 | 0.777 | −0.551 | ≥0.25 | ns |
| DPE | −0.031 | 0.022 | −1.425 | 0.158 | ns |
| Temperature32:DPE | 0.026 | 0.028 | 0.917 | ≥0.25 | ns |
| Temperature35:DPE | 0.036 | 0.028 | 1.300 | 0.197 | ns |
| Simpson | |||||
| (Intercept) | −0.282 | 0.617 | −0.456 | ≥0.25 | ns |
| Temperature32 | 0.336 | 0.801 | 0.420 | ≥0.25 | ns |
| Temperature35 | −0.172 | 0.799 | −0.215 | ≥0.25 | ns |
| DPE | −0.028 | 0.022 | −1.234 | 0.221 | ns |
| Temperature32:DPE | 0.028 | 0.029 | 0.946 | ≥0.25 | ns |
| Temperature35:DPE | 0.036 | 0.029 | 1.261 | 0.211 | ns |
| Richness | |||||
| (Intercept) | 0.316 | 0.480 | 0.657 | ≥0.25 | ns |
| Temperature32 | −0.034 | 0.636 | −0.054 | ≥0.25 | ns |
| Temperature35 | −0.596 | 0.638 | −0.934 | ≥0.25 | ns |
| DPE | −0.038 | 0.017 | −2.191 | 0.031 | * |
| Temperature32:DPE | 0.028 | 0.023 | 1.199 | 0.234 | ns |
| Temperature35:DPE | 0.032 | 0.023 | 1.405 | 0.164 | ns |
| Phylogenetic | |||||
| (Intercept) | 0.305 | 0.451 | 0.676 | ≥0.25 | ns |
| Temperature32 | −0.101 | 0.597 | −0.169 | ≥0.25 | ns |
| Temperature35 | −0.766 | 0.601 | −1.274 | 0.206 | ns |
| DPE | −0.038 | 0.016 | −2.310 | 0.023 | * |
| Temperature32:DPE | 0.028 | 0.022 | 1.298 | 0.198 | ns |
| Temperature35:DPE | 0.036 | 0.022 | 1.651 | 0.103 | ns |
| ANOVA of GLM | ||||
| ANOVA(GLM(Alpha.Score ~ Temperature*Time), type = 2); Exposed fish | ||||
| term | statistic | df | p.value | sig |
|---|---|---|---|---|
| Shannon | ||||
| Temperature | 8.789 | 2.000 | 0.012 | * |
| DPE | 0.523 | 1.000 | ≥0.25 | ns |
| Temperature:DPE | 1.759 | 2.000 | ≥0.25 | ns |
| Simpson | ||||
| Temperature | 11.871 | 2.000 | 0.003 | ** |
| DPE | 0.120 | 1.000 | ≥0.25 | ns |
| Temperature:DPE | 1.684 | 2.000 | ≥0.25 | ns |
| Richness | ||||
| Temperature | 7.911 | 2.000 | 0.019 | * |
| DPE | 3.435 | 1.000 | 0.064 | ns |
| Temperature:DPE | 2.255 | 2.000 | ≥0.25 | ns |
| Phylogenetic | ||||
| Temperature | 8.173 | 2.000 | 0.017 | * |
| DPE | 3.103 | 1.000 | 0.078 | ns |
| Temperature:DPE | 2.980 | 2.000 | 0.225 | ns |
| Pairwise Tukey's HSD, p.adj: Dunnett | ||||||||||
| Tukey(Alpha.Score ~ Temperature*Time); Exposed fish | ||||||||||
| Temperature | .y. | term | group1 | group2 | estimate | std.error | statistic | adj.p.value | Variable | Group |
|---|---|---|---|---|---|---|---|---|---|---|
| Shannon | ||||||||||
| 28 | Alpha.Score | DPE | 21 | 14 | −3.311 | 1.822 | −1.817 | 0.246 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 14 | −2.027 | 1.023 | −1.982 | 0.178 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 14 | −1.024 | 0.705 | −1.452 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 21 | 1.284 | 1.982 | 0.648 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 21 | 2.287 | 1.838 | 1.245 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 28 | 1.004 | 1.050 | 0.956 | ≥0.25 | DPE | 28 |
| 32 | Alpha.Score | DPE | 21 | 14 | −0.536 | 0.537 | −0.999 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 14 | 0.056 | 0.558 | 0.099 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 14 | −0.271 | 0.532 | −0.509 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 21 | 0.592 | 0.606 | 0.977 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 21 | 0.266 | 0.581 | 0.457 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 28 | −0.326 | 0.601 | −0.543 | ≥0.25 | DPE | 32 |
| 35 | Alpha.Score | DPE | 21 | 14 | −0.246 | 0.270 | −0.909 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 14 | −0.292 | 0.271 | −1.075 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 14 | 0.097 | 0.258 | 0.377 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 21 | −0.046 | 0.283 | −0.162 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 21 | 0.343 | 0.270 | 1.273 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 28 | 0.389 | 0.271 | 1.438 | ≥0.25 | DPE | 35 |
| Simpson | ||||||||||
| 28 | Alpha.Score | DPE | 21 | 14 | −3.141 | 1.820 | −1.725 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 14 | −1.994 | 1.072 | −1.861 | 0.227 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 14 | −0.925 | 0.721 | −1.283 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 21 | 1.147 | 2.001 | 0.573 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 21 | 2.215 | 1.837 | 1.206 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 28 | 1.069 | 1.100 | 0.971 | ≥0.25 | DPE | 28 |
| 32 | Alpha.Score | DPE | 21 | 14 | −0.416 | 0.550 | −0.756 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 14 | 0.275 | 0.580 | 0.474 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 14 | −0.138 | 0.546 | −0.252 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 21 | 0.691 | 0.626 | 1.103 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 21 | 0.278 | 0.595 | 0.467 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 28 | −0.413 | 0.623 | −0.662 | ≥0.25 | DPE | 32 |
| 35 | Alpha.Score | DPE | 21 | 14 | −0.374 | 0.297 | −1.258 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 14 | −0.285 | 0.296 | −0.966 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 14 | 0.142 | 0.284 | 0.502 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 21 | 0.088 | 0.308 | 0.287 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 21 | 0.516 | 0.297 | 1.738 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 28 | 0.428 | 0.295 | 1.448 | ≥0.25 | DPE | 35 |
| Richness | ||||||||||
| 28 | Alpha.Score | DPE | 21 | 14 | −2.219 | 1.016 | −2.185 | 0.122 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 14 | −1.585 | 0.795 | −1.993 | 0.184 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 14 | −1.239 | 0.650 | −1.907 | 0.218 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 21 | 0.634 | 1.133 | 0.560 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 21 | 0.980 | 1.036 | 0.946 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 28 | 0.346 | 0.821 | 0.421 | ≥0.25 | DPE | 28 |
| 32 | Alpha.Score | DPE | 21 | 14 | −0.561 | 0.412 | −1.363 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 14 | −0.470 | 0.430 | −1.091 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 14 | −0.357 | 0.410 | −0.871 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 21 | 0.092 | 0.462 | 0.199 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 21 | 0.204 | 0.444 | 0.460 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 28 | 0.112 | 0.461 | 0.243 | ≥0.25 | DPE | 32 |
| 35 | Alpha.Score | DPE | 21 | 14 | −0.338 | 0.242 | −1.396 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 14 | −0.329 | 0.242 | −1.361 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 14 | −0.233 | 0.233 | −1.000 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 21 | 0.009 | 0.252 | 0.035 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 21 | 0.105 | 0.244 | 0.430 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 28 | 0.096 | 0.244 | 0.394 | ≥0.25 | DPE | 35 |
| Phylogenetic | ||||||||||
| 28 | Alpha.Score | DPE | 21 | 14 | −2.030 | 0.921 | −2.205 | 0.118 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 14 | −1.472 | 0.742 | −1.985 | 0.188 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 14 | −1.216 | 0.616 | −1.975 | 0.192 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 21 | 0.557 | 1.029 | 0.542 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 21 | 0.814 | 0.942 | 0.864 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 28 | 0.256 | 0.768 | 0.334 | ≥0.25 | DPE | 28 |
| 32 | Alpha.Score | DPE | 21 | 14 | −0.537 | 0.382 | −1.406 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 14 | −0.442 | 0.399 | −1.108 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 14 | −0.329 | 0.380 | −0.866 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 21 | 0.095 | 0.430 | 0.222 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 21 | 0.208 | 0.413 | 0.504 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 28 | 0.113 | 0.428 | 0.263 | ≥0.25 | DPE | 32 |
| 35 | Alpha.Score | DPE | 21 | 14 | −0.273 | 0.236 | −1.159 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 14 | −0.214 | 0.235 | −0.911 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 14 | −0.121 | 0.226 | −0.535 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 21 | 0.059 | 0.246 | 0.242 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 21 | 0.152 | 0.237 | 0.642 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 28 | 0.093 | 0.236 | 0.393 | ≥0.25 | DPE | 35 |
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| ADONIS2 | ||||||
| adonis2(Beta Distance ~ Temperature*DPE); Exposed fish | ||||||
| term | df | SumOfSqs | R2 | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 5.218 | 0.251 | 14.843 | 0.001 | *** |
| DPE | 1.000 | 0.394 | 0.019 | 2.241 | 0.058 | ns |
| Temperature:DPE | 2.000 | 0.548 | 0.026 | 1.558 | 0.101 | ns |
| Residual | 83.000 | 14.589 | 0.703 | NA | NA | NA |
| Total | 88.000 | 20.748 | 1.000 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 3.573 | 0.117 | 5.847 | 0.001 | *** |
| DPE | 1.000 | 0.674 | 0.022 | 2.207 | 0.001 | *** |
| Temperature:DPE | 2.000 | 0.913 | 0.030 | 1.494 | 0.013 | * |
| Residual | 83.000 | 25.360 | 0.831 | NA | NA | NA |
| Total | 88.000 | 30.520 | 1.000 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 3.363 | 0.212 | 11.847 | 0.001 | *** |
| DPE | 1.000 | 0.261 | 0.016 | 1.837 | 0.101 | ns |
| Temperature:DPE | 2.000 | 0.436 | 0.028 | 1.535 | 0.122 | ns |
| Residual | 83.000 | 11.781 | 0.744 | NA | NA | NA |
| Total | 88.000 | 15.841 | 1.000 | NA | NA | NA |
| ANOVA: Homogeneity of Dispersion | ||||||
| ANOVA(Beta Disperson ~ Temperature); Exposed fish | ||||||
| term | df | sumsq | meansq | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temp.DPE | 11.000 | 0.624 | 0.057 | 3.261 | 0.001 | ** |
| Residual | 77.000 | 1.339 | 0.017 | NA | NA | NA |
| canberra | ||||||
| Temp.DPE | 11.000 | 0.316 | 0.029 | 2.748 | 0.005 | ** |
| Residual | 77.000 | 0.805 | 0.010 | NA | NA | NA |
| gunifrac | ||||||
| Temp.DPE | 11.000 | 0.420 | 0.038 | 3.232 | 0.001 | ** |
| Residual | 77.000 | 0.909 | 0.012 | NA | NA | NA |
| Tukey: Homogeneity of Dispersion | ||||||||
| Tukey(Beta Disperson ~ Temperature*DPE); Exposed fish | ||||||||
| .y. | term | group1 | group2 | estimate | conf.low | conf.high | adj.p.value | sig |
|---|---|---|---|---|---|---|---|---|
| bray | ||||||||
| Distance | Temp.DPE | 28°C__21DPE | 28°C__14DPE | −0.213 | −0.485 | 0.059 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__14DPE | −0.258 | −0.512 | −0.005 | 0.042 | * |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__14DPE | −0.063 | −0.285 | 0.160 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__14DPE | −0.059 | −0.270 | 0.152 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__14DPE | −0.089 | −0.319 | 0.141 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__14DPE | −0.123 | −0.363 | 0.117 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__14DPE | −0.142 | −0.372 | 0.088 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__14DPE | −0.202 | −0.418 | 0.014 | 0.090 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__14DPE | −0.170 | −0.393 | 0.052 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__14DPE | −0.289 | −0.511 | −0.067 | 0.002 | ** |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__14DPE | −0.192 | −0.408 | 0.024 | 0.130 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__21DPE | −0.046 | −0.344 | 0.253 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__21DPE | 0.150 | −0.122 | 0.422 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__21DPE | 0.153 | −0.110 | 0.416 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__21DPE | 0.124 | −0.154 | 0.403 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__21DPE | 0.090 | −0.197 | 0.377 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__21DPE | 0.071 | −0.208 | 0.350 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__21DPE | 0.011 | −0.256 | 0.278 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__21DPE | 0.042 | −0.230 | 0.315 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__21DPE | −0.077 | −0.349 | 0.196 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__21DPE | 0.021 | −0.246 | 0.288 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__28DPE | 0.196 | −0.058 | 0.449 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__28DPE | 0.199 | −0.044 | 0.443 | 0.220 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__28DPE | 0.170 | −0.091 | 0.430 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__28DPE | 0.136 | −0.133 | 0.405 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__28DPE | 0.117 | −0.144 | 0.377 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__28DPE | 0.057 | −0.191 | 0.305 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__28DPE | 0.088 | −0.165 | 0.341 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__28DPE | −0.031 | −0.284 | 0.222 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__28DPE | 0.066 | −0.182 | 0.314 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__42DPE | 0.003 | −0.207 | 0.214 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__42DPE | −0.026 | −0.256 | 0.204 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__42DPE | −0.060 | −0.300 | 0.180 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__42DPE | −0.079 | −0.309 | 0.151 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__42DPE | −0.139 | −0.355 | 0.077 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__42DPE | −0.108 | −0.330 | 0.115 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__42DPE | −0.227 | −0.449 | −0.004 | 0.042 | * |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__42DPE | −0.129 | −0.345 | 0.087 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__14DPE | −0.029 | −0.248 | 0.190 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__14DPE | −0.063 | −0.293 | 0.166 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__14DPE | −0.082 | −0.301 | 0.137 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__14DPE | −0.142 | −0.347 | 0.062 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__14DPE | −0.111 | −0.322 | 0.100 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__14DPE | −0.230 | −0.441 | −0.019 | 0.021 | * |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__14DPE | −0.133 | −0.337 | 0.072 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__21DPE | −0.034 | −0.281 | 0.213 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__21DPE | −0.053 | −0.291 | 0.185 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__21DPE | −0.113 | −0.337 | 0.111 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__21DPE | −0.082 | −0.312 | 0.148 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__21DPE | −0.201 | −0.431 | 0.029 | 0.148 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__21DPE | −0.103 | −0.327 | 0.121 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__28DPE | −0.019 | −0.266 | 0.228 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__28DPE | −0.079 | −0.313 | 0.155 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__28DPE | −0.048 | −0.288 | 0.192 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__28DPE | −0.167 | −0.407 | 0.073 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__28DPE | −0.069 | −0.304 | 0.165 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__42DPE | −0.060 | −0.284 | 0.164 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__42DPE | −0.029 | −0.259 | 0.201 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__42DPE | −0.148 | −0.378 | 0.082 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__42DPE | −0.050 | −0.274 | 0.174 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__14DPE | 0.031 | −0.185 | 0.247 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__14DPE | −0.088 | −0.304 | 0.128 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__14DPE | 0.010 | −0.200 | 0.219 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__21DPE | −0.119 | −0.341 | 0.103 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__21DPE | −0.022 | −0.238 | 0.194 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__28DPE | 0.097 | −0.119 | 0.313 | ≥0.25 | ns |
| canberra | ||||||||
| Distance | Temp.DPE | 28°C__21DPE | 28°C__14DPE | −0.004 | −0.215 | 0.207 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__14DPE | 0.040 | −0.156 | 0.237 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__14DPE | 0.059 | −0.113 | 0.232 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__14DPE | −0.043 | −0.206 | 0.121 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__14DPE | −0.044 | −0.223 | 0.134 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__14DPE | −0.102 | −0.288 | 0.084 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__14DPE | −0.084 | −0.262 | 0.095 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__14DPE | −0.097 | −0.265 | 0.070 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__14DPE | −0.097 | −0.269 | 0.075 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__14DPE | −0.142 | −0.314 | 0.031 | 0.215 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__14DPE | −0.103 | −0.271 | 0.064 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__21DPE | 0.044 | −0.187 | 0.276 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__21DPE | 0.064 | −0.148 | 0.275 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__21DPE | −0.039 | −0.243 | 0.165 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__21DPE | −0.040 | −0.256 | 0.176 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__21DPE | −0.097 | −0.320 | 0.125 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__21DPE | −0.079 | −0.295 | 0.137 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__21DPE | −0.093 | −0.300 | 0.114 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__21DPE | −0.093 | −0.304 | 0.118 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__21DPE | −0.137 | −0.348 | 0.074 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__21DPE | −0.099 | −0.306 | 0.108 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__28DPE | 0.019 | −0.177 | 0.216 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__28DPE | −0.083 | −0.272 | 0.106 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__28DPE | −0.085 | −0.286 | 0.117 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__28DPE | −0.142 | −0.351 | 0.067 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__28DPE | −0.124 | −0.326 | 0.078 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__28DPE | −0.137 | −0.329 | 0.055 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__28DPE | −0.137 | −0.334 | 0.059 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__28DPE | −0.182 | −0.378 | 0.015 | 0.097 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__28DPE | −0.144 | −0.336 | 0.049 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__42DPE | −0.102 | −0.266 | 0.061 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__42DPE | −0.104 | −0.282 | 0.075 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__42DPE | −0.161 | −0.347 | 0.025 | 0.157 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__42DPE | −0.143 | −0.321 | 0.036 | 0.247 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__42DPE | −0.156 | −0.324 | 0.011 | 0.091 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__42DPE | −0.156 | −0.329 | 0.016 | 0.112 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__42DPE | −0.201 | −0.373 | −0.028 | 0.010 | ** |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__42DPE | −0.163 | −0.330 | 0.005 | 0.065 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__14DPE | −0.001 | −0.171 | 0.168 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__14DPE | −0.059 | −0.237 | 0.119 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__14DPE | −0.041 | −0.211 | 0.129 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__14DPE | −0.054 | −0.212 | 0.104 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__14DPE | −0.054 | −0.218 | 0.109 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__14DPE | −0.099 | −0.262 | 0.065 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__14DPE | −0.060 | −0.219 | 0.098 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__21DPE | −0.057 | −0.249 | 0.134 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__21DPE | −0.039 | −0.224 | 0.145 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__21DPE | −0.053 | −0.226 | 0.121 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__21DPE | −0.053 | −0.231 | 0.126 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__21DPE | −0.097 | −0.276 | 0.081 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__21DPE | −0.059 | −0.233 | 0.115 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__28DPE | 0.018 | −0.174 | 0.210 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__28DPE | 0.005 | −0.177 | 0.186 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__28DPE | 0.005 | −0.182 | 0.191 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__28DPE | −0.040 | −0.226 | 0.146 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__28DPE | −0.002 | −0.183 | 0.180 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__42DPE | −0.013 | −0.187 | 0.160 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__42DPE | −0.014 | −0.192 | 0.165 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__42DPE | −0.058 | −0.236 | 0.120 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__42DPE | −0.020 | −0.194 | 0.154 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__14DPE | 0.000 | −0.168 | 0.167 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__14DPE | −0.044 | −0.212 | 0.123 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__14DPE | −0.006 | −0.169 | 0.156 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__21DPE | −0.044 | −0.217 | 0.128 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__21DPE | −0.006 | −0.174 | 0.161 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__28DPE | 0.038 | −0.129 | 0.206 | ≥0.25 | ns |
| gunifrac | ||||||||
| Distance | Temp.DPE | 28°C__21DPE | 28°C__14DPE | −0.191 | −0.415 | 0.033 | 0.173 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__14DPE | −0.061 | −0.270 | 0.147 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__14DPE | 0.018 | −0.165 | 0.201 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__14DPE | −0.024 | −0.197 | 0.150 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__14DPE | −0.027 | −0.216 | 0.163 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__14DPE | −0.100 | −0.298 | 0.098 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__14DPE | −0.069 | −0.259 | 0.120 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__14DPE | −0.131 | −0.309 | 0.047 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__14DPE | −0.137 | −0.320 | 0.046 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__14DPE | −0.198 | −0.382 | −0.015 | 0.022 | * |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__14DPE | −0.134 | −0.312 | 0.044 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__21DPE | 0.130 | −0.116 | 0.375 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__21DPE | 0.209 | −0.015 | 0.433 | 0.092 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__21DPE | 0.167 | −0.049 | 0.384 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__21DPE | 0.164 | −0.065 | 0.394 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__21DPE | 0.091 | −0.146 | 0.327 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__21DPE | 0.122 | −0.108 | 0.351 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__21DPE | 0.060 | −0.161 | 0.280 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__21DPE | 0.054 | −0.170 | 0.278 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__21DPE | −0.007 | −0.232 | 0.217 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__21DPE | 0.057 | −0.163 | 0.277 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__28DPE | 0.079 | −0.130 | 0.288 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__28DPE | 0.038 | −0.163 | 0.238 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__28DPE | 0.035 | −0.180 | 0.249 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__28DPE | −0.039 | −0.260 | 0.183 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__28DPE | −0.008 | −0.222 | 0.206 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__28DPE | −0.070 | −0.274 | 0.134 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__28DPE | −0.075 | −0.284 | 0.133 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__28DPE | −0.137 | −0.346 | 0.072 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__28DPE | −0.072 | −0.277 | 0.132 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__42DPE | −0.041 | −0.215 | 0.132 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__42DPE | −0.045 | −0.234 | 0.145 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__42DPE | −0.118 | −0.316 | 0.080 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__42DPE | −0.087 | −0.277 | 0.102 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__42DPE | −0.149 | −0.327 | 0.029 | 0.190 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__42DPE | −0.155 | −0.338 | 0.029 | 0.182 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__42DPE | −0.216 | −0.399 | −0.033 | 0.008 | ** |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__42DPE | −0.151 | −0.329 | 0.026 | 0.173 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__14DPE | −0.003 | −0.184 | 0.177 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__14DPE | −0.076 | −0.266 | 0.113 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__14DPE | −0.046 | −0.226 | 0.135 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__14DPE | −0.108 | −0.276 | 0.060 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__14DPE | −0.113 | −0.287 | 0.061 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__14DPE | −0.175 | −0.348 | −0.001 | 0.047 | * |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__14DPE | −0.110 | −0.278 | 0.058 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__21DPE | −0.073 | −0.277 | 0.130 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__21DPE | −0.043 | −0.238 | 0.153 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__21DPE | −0.105 | −0.289 | 0.080 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__21DPE | −0.110 | −0.300 | 0.079 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__21DPE | −0.172 | −0.361 | 0.018 | 0.113 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__21DPE | −0.107 | −0.292 | 0.078 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__28DPE | 0.031 | −0.173 | 0.234 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__28DPE | −0.031 | −0.224 | 0.162 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__28DPE | −0.037 | −0.234 | 0.161 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__28DPE | −0.098 | −0.296 | 0.099 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__28DPE | −0.034 | −0.227 | 0.159 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__42DPE | −0.062 | −0.247 | 0.122 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__42DPE | −0.067 | −0.257 | 0.122 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__42DPE | −0.129 | −0.319 | 0.060 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__42DPE | −0.064 | −0.249 | 0.120 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__14DPE | −0.005 | −0.183 | 0.173 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__14DPE | −0.067 | −0.245 | 0.111 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__14DPE | −0.002 | −0.175 | 0.170 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__21DPE | −0.062 | −0.245 | 0.121 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__21DPE | 0.003 | −0.175 | 0.181 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__28DPE | 0.065 | −0.113 | 0.243 | ≥0.25 | ns |
Click on tabs to reveal supplementary figures and tables.
Fig. 5 Exposure to Pseudocapillaria tomentosa differentially impacts gut microbiome depending on water temperature. (A) Simpson’s Index for diversity of parasite exposed fish. Gut microbial alpha-diversity does not significantly differ between fish reared at the same water temperature with presence or absence of infection. (B) Capscale ordination based on the Canberra dissimilarity of gut microbiome composition of parasite exposed fish. The analysis shows that gut microbiome composition significantly differs between positively infected fish reared at different water temperatures. (C) Total worm count is positively correlated with lowest or highest alpha diversity scores in fish with detectable parasites. Points are colored by temperature and filled by cluster (High = purple, Low = orange, Other = white), and samples with a worm count equal to 0 are transparent. (D) Capscale ordination based on the Bray-Curtis dissimilarity of gut microbiome composition of water temperature and worm counts. Points are colored by temperature and filled by cluster (High = purple, Low = orange, Other = white), and samples with a worm count equal to 0 are transparent. The analysis shows that gut microbiome composition significantly differs between water temperature and total worm count, and samples labeled as “High” or “Low” form distinct clusters. Ribbons and ellipses indicate 95% confidence interval. Only statistically significant relationships are shown. A “*” indicates statistical significance below the “0.05” level. Black arrows indicate statistically significant covariates and direction of greatest change in the indicated covariates.
Click on tabs to display tables. Scroll to see additional rows.
| GLM Results | |||||
| glm(Alpha.Score ~ Temperature*Pathology.Results); Exposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| Shannon | |||||
| (Intercept) | −1.090 | 0.429 | −2.544 | 0.013 | * |
| Temperature32 | 0.889 | 0.508 | 1.749 | 0.084 | ns |
| Temperature35 | 0.693 | 0.468 | 1.482 | 0.142 | ns |
| Pathology.Resultspositive | 0.223 | 0.512 | 0.436 | ≥0.25 | ns |
| Temperature32:Pathology.Resultspositive | 0.125 | 0.641 | 0.195 | ≥0.25 | ns |
| Temperature35:Pathology.Resultspositive | −1.122 | 1.392 | −0.806 | ≥0.25 | ns |
| Simpson | |||||
| (Intercept) | −1.265 | 0.458 | −2.760 | 0.007 | ** |
| Temperature32 | 1.105 | 0.536 | 2.061 | 0.042 | * |
| Temperature35 | 1.055 | 0.495 | 2.130 | 0.036 | * |
| Pathology.Resultspositive | 0.375 | 0.541 | 0.694 | ≥0.25 | ns |
| Temperature32:Pathology.Resultspositive | 0.054 | 0.669 | 0.081 | ≥0.25 | ns |
| Temperature35:Pathology.Resultspositive | −1.154 | 1.337 | −0.863 | ≥0.25 | ns |
| Richness | |||||
| (Intercept) | −0.421 | 0.329 | −1.279 | 0.204 | ns |
| Temperature32 | 0.483 | 0.405 | 1.194 | 0.236 | ns |
| Temperature35 | −0.002 | 0.367 | −0.005 | ≥0.25 | ns |
| Pathology.Resultspositive | −0.390 | 0.407 | −0.957 | ≥0.25 | ns |
| Temperature32:Pathology.Resultspositive | 0.310 | 0.526 | 0.589 | ≥0.25 | ns |
| Temperature35:Pathology.Resultspositive | −0.221 | 1.125 | −0.197 | ≥0.25 | ns |
| Phylogenetic | |||||
| (Intercept) | −0.421 | 0.311 | −1.353 | 0.180 | ns |
| Temperature32 | 0.397 | 0.383 | 1.037 | ≥0.25 | ns |
| Temperature35 | −0.085 | 0.348 | −0.244 | ≥0.25 | ns |
| Pathology.Resultspositive | −0.390 | 0.385 | −1.014 | ≥0.25 | ns |
| Temperature32:Pathology.Resultspositive | 0.373 | 0.497 | 0.750 | ≥0.25 | ns |
| Temperature35:Pathology.Resultspositive | −0.112 | 1.058 | −0.105 | ≥0.25 | ns |
| ANOVA of GLM | ||||
| ANOVA(GLM(Alpha.Score ~ Temperature*Time), type = 2); Exposed fish | ||||
| term | statistic | df | p.value | sig |
|---|---|---|---|---|
| Shannon | ||||
| Temperature | 9.895 | 2.000 | 0.007 | ** |
| Pathology.Results | 0.569 | 1.000 | ≥0.25 | ns |
| Temperature:Pathology.Results | 0.997 | 2.000 | ≥0.25 | ns |
| Simpson | ||||
| Temperature | 13.663 | 2.000 | 0.001 | ** |
| Pathology.Results | 1.134 | 1.000 | ≥0.25 | ns |
| Temperature:Pathology.Results | 0.999 | 2.000 | ≥0.25 | ns |
| Richness | ||||
| Temperature | 8.625 | 2.000 | 0.013 | * |
| Pathology.Results | 0.840 | 1.000 | ≥0.25 | ns |
| Temperature:Pathology.Results | 0.495 | 2.000 | ≥0.25 | ns |
| Phylogenetic | ||||
| Temperature | 8.874 | 2.000 | 0.012 | * |
| Pathology.Results | 0.625 | 1.000 | ≥0.25 | ns |
| Temperature:Pathology.Results | 0.674 | 2.000 | ≥0.25 | ns |
| Pairwise Tukey's HSD, p.adj: Dunnett | ||||||||||
| Tukey(Alpha.Score ~ Temperature*Time); Exposed fish | ||||||||||
| Temperature | .y. | term | group1 | group2 | estimate | std.error | statistic | adj.p.value | Variable | Group |
|---|---|---|---|---|---|---|---|---|---|---|
| Shannon | ||||||||||
| 28 | Alpha.Score | Pathology.Results | positive | negative | 0.223 | 0.732 | 0.305 | ≥0.25 | Pathology.Results | 28 |
| 32 | Alpha.Score | Pathology.Results | positive | negative | 0.348 | 0.383 | 0.908 | ≥0.25 | Pathology.Results | 32 |
| 35 | Alpha.Score | Pathology.Results | positive | negative | −0.899 | 0.659 | −1.365 | 0.172 | Pathology.Results | 35 |
| Simpson | ||||||||||
| 28 | Alpha.Score | Pathology.Results | positive | negative | 0.375 | 0.754 | 0.497 | ≥0.25 | Pathology.Results | 28 |
| 32 | Alpha.Score | Pathology.Results | positive | negative | 0.429 | 0.394 | 1.089 | ≥0.25 | Pathology.Results | 32 |
| 35 | Alpha.Score | Pathology.Results | positive | negative | −0.779 | 0.692 | −1.125 | ≥0.25 | Pathology.Results | 35 |
| Richness | ||||||||||
| 28 | Alpha.Score | Pathology.Results | positive | negative | −0.390 | 0.608 | −0.641 | ≥0.25 | Pathology.Results | 28 |
| 32 | Alpha.Score | Pathology.Results | positive | negative | −0.080 | 0.301 | −0.267 | ≥0.25 | Pathology.Results | 32 |
| 35 | Alpha.Score | Pathology.Results | positive | negative | −0.611 | 0.552 | −1.107 | ≥0.25 | Pathology.Results | 35 |
| Phylogenetic | ||||||||||
| 28 | Alpha.Score | Pathology.Results | positive | negative | −0.390 | 0.577 | −0.676 | ≥0.25 | Pathology.Results | 28 |
| 32 | Alpha.Score | Pathology.Results | positive | negative | −0.017 | 0.280 | −0.062 | ≥0.25 | Pathology.Results | 32 |
| 35 | Alpha.Score | Pathology.Results | positive | negative | −0.502 | 0.524 | −0.958 | ≥0.25 | Pathology.Results | 35 |
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| ADONIS2 | ||||||
| adonis2(Beta Distance ~ Temperature*Pathology.Results); Exposed fish | ||||||
| term | df | SumOfSqs | R2 | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 5.218 | 0.251 | 14.569 | 0.001 | *** |
| Pathology.Results | 1.000 | 0.263 | 0.013 | 1.471 | 0.175 | ns |
| Temperature:Pathology.Results | 2.000 | 0.404 | 0.019 | 1.127 | ≥0.25 | ns |
| Residual | 83.000 | 14.863 | 0.716 | NA | NA | NA |
| Total | 88.000 | 20.748 | 1.000 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 3.573 | 0.117 | 5.751 | 0.001 | *** |
| Pathology.Results | 1.000 | 0.488 | 0.016 | 1.570 | 0.033 | * |
| Temperature:Pathology.Results | 2.000 | 0.679 | 0.022 | 1.093 | ≥0.25 | ns |
| Residual | 83.000 | 25.781 | 0.845 | NA | NA | NA |
| Total | 88.000 | 30.520 | 1.000 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 3.363 | 0.212 | 11.651 | 0.001 | *** |
| Pathology.Results | 1.000 | 0.234 | 0.015 | 1.622 | 0.128 | ns |
| Temperature:Pathology.Results | 2.000 | 0.264 | 0.017 | 0.915 | ≥0.25 | ns |
| Residual | 83.000 | 11.980 | 0.756 | NA | NA | NA |
| Total | 88.000 | 15.841 | 1.000 | NA | NA | NA |
| ANOVA: Homogeneity of Dispersion | ||||||
| ANOVA(Beta Disperson ~ Temperature*Pathology.Results); Exposed fish | ||||||
| term | df | sumsq | meansq | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temp.Path | 5.000 | 0.520 | 0.104 | 4.652 | <0.001 | *** |
| Residual | 83.000 | 1.855 | 0.022 | NA | NA | NA |
| canberra | ||||||
| Temp.Path | 5.000 | 0.625 | 0.125 | 13.830 | <0.001 | **** |
| Residual | 83.000 | 0.750 | 0.009 | NA | NA | NA |
| gunifrac | ||||||
| Temp.Path | 5.000 | 0.436 | 0.087 | 6.954 | <0.001 | **** |
| Residual | 83.000 | 1.040 | 0.013 | NA | NA | NA |
| Tukey: Homogeneity of Dispersion | ||||||||
| Tukey(Beta Disperson ~ Temperature*Pathology.Results); Exposed fish | ||||||||
| .y. | term | group1 | group2 | estimate | conf.low | conf.high | adj.p.value | sig |
|---|---|---|---|---|---|---|---|---|
| bray | ||||||||
| Distance | Temp.Path | 28°C__pos | 28°C__neg | 0.022 | −0.165 | 0.209 | ≥0.25 | ns |
| Distance | Temp.Path | 32°C__neg | 28°C__neg | −0.086 | −0.277 | 0.105 | ≥0.25 | ns |
| Distance | Temp.Path | 32°C__pos | 28°C__neg | 0.015 | −0.176 | 0.206 | ≥0.25 | ns |
| Distance | Temp.Path | 35°C__neg | 28°C__neg | −0.126 | −0.298 | 0.046 | ≥0.25 | ns |
| Distance | Temp.Path | 35°C__pos | 28°C__neg | −0.434 | −0.896 | 0.029 | 0.079 | ns |
| Distance | Temp.Path | 32°C__neg | 28°C__pos | −0.108 | −0.263 | 0.046 | ≥0.25 | ns |
| Distance | Temp.Path | 32°C__pos | 28°C__pos | −0.007 | −0.161 | 0.148 | ≥0.25 | ns |
| Distance | Temp.Path | 35°C__neg | 28°C__pos | −0.148 | −0.278 | −0.017 | 0.017 | * |
| Distance | Temp.Path | 35°C__pos | 28°C__pos | −0.456 | −0.905 | −0.007 | 0.044 | * |
| Distance | Temp.Path | 32°C__pos | 32°C__neg | 0.101 | −0.058 | 0.261 | ≥0.25 | ns |
| Distance | Temp.Path | 35°C__neg | 32°C__neg | −0.039 | −0.175 | 0.096 | ≥0.25 | ns |
| Distance | Temp.Path | 35°C__pos | 32°C__neg | −0.348 | −0.798 | 0.103 | 0.226 | ns |
| Distance | Temp.Path | 35°C__neg | 32°C__pos | −0.141 | −0.277 | −0.005 | 0.038 | * |
| Distance | Temp.Path | 35°C__pos | 32°C__pos | −0.449 | −0.899 | 0.001 | 0.051 | ns |
| Distance | Temp.Path | 35°C__pos | 35°C__neg | −0.308 | −0.751 | 0.135 | ≥0.25 | ns |
| canberra | ||||||||
| Distance | Temp.Path | 28°C__pos | 28°C__neg | 0.045 | −0.074 | 0.164 | ≥0.25 | ns |
| Distance | Temp.Path | 32°C__neg | 28°C__neg | −0.123 | −0.244 | −0.002 | 0.045 | * |
| Distance | Temp.Path | 32°C__pos | 28°C__neg | −0.042 | −0.164 | 0.079 | ≥0.25 | ns |
| Distance | Temp.Path | 35°C__neg | 28°C__neg | −0.105 | −0.214 | 0.005 | 0.068 | ns |
| Distance | Temp.Path | 35°C__pos | 28°C__neg | −0.588 | −0.883 | −0.294 | <0.001 | **** |
| Distance | Temp.Path | 32°C__neg | 28°C__pos | −0.168 | −0.267 | −0.070 | <0.001 | **** |
| Distance | Temp.Path | 32°C__pos | 28°C__pos | −0.088 | −0.186 | 0.010 | 0.107 | ns |
| Distance | Temp.Path | 35°C__neg | 28°C__pos | −0.150 | −0.233 | −0.067 | <0.001 | **** |
| Distance | Temp.Path | 35°C__pos | 28°C__pos | −0.634 | −0.919 | −0.348 | <0.001 | **** |
| Distance | Temp.Path | 32°C__pos | 32°C__neg | 0.080 | −0.021 | 0.182 | 0.199 | ns |
| Distance | Temp.Path | 35°C__neg | 32°C__neg | 0.018 | −0.068 | 0.105 | ≥0.25 | ns |
| Distance | Temp.Path | 35°C__pos | 32°C__neg | −0.465 | −0.752 | −0.179 | <0.001 | *** |
| Distance | Temp.Path | 35°C__neg | 32°C__pos | −0.062 | −0.149 | 0.024 | ≥0.25 | ns |
| Distance | Temp.Path | 35°C__pos | 32°C__pos | −0.546 | −0.832 | −0.259 | <0.001 | **** |
| Distance | Temp.Path | 35°C__pos | 35°C__neg | −0.484 | −0.765 | −0.202 | <0.001 | **** |
| gunifrac | ||||||||
| Distance | Temp.Path | 28°C__pos | 28°C__neg | 0.011 | −0.129 | 0.151 | ≥0.25 | ns |
| Distance | Temp.Path | 32°C__neg | 28°C__neg | −0.091 | −0.234 | 0.052 | ≥0.25 | ns |
| Distance | Temp.Path | 32°C__pos | 28°C__neg | −0.014 | −0.157 | 0.129 | ≥0.25 | ns |
| Distance | Temp.Path | 35°C__neg | 28°C__neg | −0.129 | −0.258 | 0.000 | 0.049 | * |
| Distance | Temp.Path | 35°C__pos | 28°C__neg | −0.411 | −0.758 | −0.065 | 0.011 | * |
| Distance | Temp.Path | 32°C__neg | 28°C__pos | −0.102 | −0.218 | 0.014 | 0.115 | ns |
| Distance | Temp.Path | 32°C__pos | 28°C__pos | −0.025 | −0.141 | 0.091 | ≥0.25 | ns |
| Distance | Temp.Path | 35°C__neg | 28°C__pos | −0.140 | −0.238 | −0.043 | <0.001 | *** |
| Distance | Temp.Path | 35°C__pos | 28°C__pos | −0.423 | −0.759 | −0.087 | 0.006 | ** |
| Distance | Temp.Path | 32°C__pos | 32°C__neg | 0.077 | −0.042 | 0.196 | ≥0.25 | ns |
| Distance | Temp.Path | 35°C__neg | 32°C__neg | −0.038 | −0.140 | 0.064 | ≥0.25 | ns |
| Distance | Temp.Path | 35°C__pos | 32°C__neg | −0.321 | −0.658 | 0.017 | 0.072 | ns |
| Distance | Temp.Path | 35°C__neg | 32°C__pos | −0.115 | −0.217 | −0.013 | 0.017 | * |
| Distance | Temp.Path | 35°C__pos | 32°C__pos | −0.398 | −0.735 | −0.060 | 0.011 | * |
| Distance | Temp.Path | 35°C__pos | 35°C__neg | −0.283 | −0.614 | 0.049 | 0.140 | ns |
Click on tabs to display tables. Scroll to see additional rows.
| GLM Results | |||||
| glm(Total.Worm.Count ~ Alpha.Score*Cluster); Exposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| Shannon | |||||
| (Intercept) | 0.000 | 0.778 | 0.000 | ≥0.25 | ns |
| Alpha.Score | 0.000 | 1.808 | 0.000 | ≥0.25 | ns |
| ClusterLow | 6.524 | 1.067 | 6.115 | <0.001 | **** |
| ClusterHigh | −2.610 | 3.512 | −0.743 | ≥0.25 | ns |
| Alpha.Score:ClusterLow | −10.090 | 4.999 | −2.018 | 0.047 | * |
| Alpha.Score:ClusterHigh | 13.706 | 4.766 | 2.876 | 0.005 | ** |
| Simpson | |||||
| (Intercept) | 0.000 | 0.741 | 0.000 | ≥0.25 | ns |
| Alpha.Score | 0.000 | 1.605 | 0.000 | ≥0.25 | ns |
| ClusterLow | 6.842 | 1.041 | 6.573 | <0.001 | **** |
| ClusterHigh | −3.247 | 3.900 | −0.833 | ≥0.25 | ns |
| Alpha.Score:ClusterLow | −12.165 | 4.647 | −2.618 | 0.011 | * |
| Alpha.Score:ClusterHigh | 14.198 | 5.090 | 2.789 | 0.007 | ** |
| Richness | |||||
| (Intercept) | 0.000 | 0.914 | 0.000 | ≥0.25 | ns |
| Alpha.Score | 0.000 | 1.983 | 0.000 | ≥0.25 | ns |
| ClusterLow | 6.105 | 1.294 | 4.717 | <0.001 | **** |
| ClusterHigh | 7.362 | 2.644 | 2.784 | 0.007 | ** |
| Alpha.Score:ClusterLow | −3.491 | 4.463 | −0.782 | ≥0.25 | ns |
| Alpha.Score:ClusterHigh | 0.772 | 4.163 | 0.185 | ≥0.25 | ns |
| Phylogenetic | |||||
| (Intercept) | 0.000 | 0.912 | 0.000 | ≥0.25 | ns |
| Alpha.Score | 0.000 | 2.061 | 0.000 | ≥0.25 | ns |
| ClusterLow | 6.230 | 1.346 | 4.629 | <0.001 | **** |
| ClusterHigh | 8.137 | 2.589 | 3.143 | 0.002 | ** |
| Alpha.Score:ClusterLow | −3.872 | 4.662 | −0.831 | ≥0.25 | ns |
| Alpha.Score:ClusterHigh | −0.429 | 4.237 | −0.101 | ≥0.25 | ns |
| ANOVA of GLM | ||||
| ANOVA(GLM(Total.Worm.Count ~ Alpha.Score*Cluster), type = 2); Exposed fish | ||||
| term | statistic | df | p.value | sig |
|---|---|---|---|---|
| Shannon | ||||
| Alpha.Score | 0.143 | 1.000 | ≥0.25 | ns |
| Cluster | 162.212 | 2.000 | <0.001 | **** |
| Alpha.Score:Cluster | 14.204 | 2.000 | <0.001 | *** |
| Simpson | ||||
| Alpha.Score | 0.002 | 1.000 | ≥0.25 | ns |
| Cluster | 168.513 | 2.000 | <0.001 | **** |
| Alpha.Score:Cluster | 16.419 | 2.000 | <0.001 | *** |
| Richness | ||||
| Alpha.Score | 0.066 | 1.000 | ≥0.25 | ns |
| Cluster | 145.539 | 2.000 | <0.001 | **** |
| Alpha.Score:Cluster | 0.741 | 2.000 | ≥0.25 | ns |
| Phylogenetic | ||||
| Alpha.Score | 0.175 | 1.000 | ≥0.25 | ns |
| Cluster | 145.520 | 2.000 | <0.001 | **** |
| Alpha.Score:Cluster | 0.696 | 2.000 | ≥0.25 | ns |
| Pairwise Tukey's HSD, p.adj: Dunnett | ||||||||||
| Tukey(Total.Worm.Count ~ Alpha.Score*Cluster); Exposed fish | ||||||||||
| Temperature | .y. | term | group1 | group2 | estimate | std.error | statistic | adj.p.value | Variable | Group |
|---|---|---|---|---|---|---|---|---|---|---|
| Shannon | ||||||||||
| 28 | Alpha.Score | Cluster | Low | Other | −1.594 | 0.642 | −2.483 | 0.034 | Cluster | 28 |
| 28 | Alpha.Score | Cluster | High | Other | 2.844 | 0.672 | 4.233 | <0.001 | Cluster | 28 |
| 28 | Alpha.Score | Cluster | High | Low | 4.438 | 0.773 | 5.741 | <0.001 | Cluster | 28 |
| 32 | Alpha.Score | Cluster | Low | Other | −1.391 | 0.495 | −2.808 | 0.013 | Cluster | 32 |
| 32 | Alpha.Score | Cluster | High | Other | 1.147 | 0.333 | 3.443 | 0.002 | Cluster | 32 |
| 32 | Alpha.Score | Cluster | High | Low | 2.538 | 0.528 | 4.811 | <0.001 | Cluster | 32 |
| 35 | Alpha.Score | Cluster | Low | Other | −0.899 | 0.659 | −1.365 | 0.172 | Cluster | 35 |
| Simpson | ||||||||||
| 28 | Alpha.Score | Cluster | Low | Other | −1.487 | 0.656 | −2.266 | 0.060 | Cluster | 28 |
| 28 | Alpha.Score | Cluster | High | Other | 2.970 | 0.665 | 4.466 | <0.001 | Cluster | 28 |
| 28 | Alpha.Score | Cluster | High | Low | 4.457 | 0.767 | 5.810 | <0.001 | Cluster | 28 |
| 32 | Alpha.Score | Cluster | Low | Other | −1.233 | 0.487 | −2.531 | 0.029 | Cluster | 32 |
| 32 | Alpha.Score | Cluster | High | Other | 1.264 | 0.354 | 3.573 | <0.001 | Cluster | 32 |
| 32 | Alpha.Score | Cluster | High | Low | 2.497 | 0.528 | 4.729 | <0.001 | Cluster | 32 |
| 35 | Alpha.Score | Cluster | Low | Other | −0.779 | 0.692 | −1.125 | ≥0.25 | Cluster | 35 |
| Richness | ||||||||||
| 28 | Alpha.Score | Cluster | Low | Other | −1.430 | 0.589 | −2.429 | 0.040 | Cluster | 28 |
| 28 | Alpha.Score | Cluster | High | Other | 1.367 | 0.653 | 2.095 | 0.090 | Cluster | 28 |
| 28 | Alpha.Score | Cluster | High | Low | 2.797 | 0.692 | 4.043 | <0.001 | Cluster | 28 |
| 32 | Alpha.Score | Cluster | Low | Other | −1.183 | 0.387 | −3.052 | 0.006 | Cluster | 32 |
| 32 | Alpha.Score | Cluster | High | Other | 0.429 | 0.278 | 1.543 | ≥0.25 | Cluster | 32 |
| 32 | Alpha.Score | Cluster | High | Low | 1.611 | 0.410 | 3.934 | <0.001 | Cluster | 32 |
| 35 | Alpha.Score | Cluster | Low | Other | −0.611 | 0.552 | −1.107 | ≥0.25 | Cluster | 35 |
| Phylogenetic | ||||||||||
| 28 | Alpha.Score | Cluster | Low | Other | −1.315 | 0.550 | −2.389 | 0.044 | Cluster | 28 |
| 28 | Alpha.Score | Cluster | High | Other | 1.204 | 0.611 | 1.970 | 0.119 | Cluster | 28 |
| 28 | Alpha.Score | Cluster | High | Low | 2.519 | 0.638 | 3.946 | <0.001 | Cluster | 28 |
| 32 | Alpha.Score | Cluster | Low | Other | −0.994 | 0.360 | −2.760 | 0.016 | Cluster | 32 |
| 32 | Alpha.Score | Cluster | High | Other | 0.437 | 0.262 | 1.667 | 0.214 | Cluster | 32 |
| 32 | Alpha.Score | Cluster | High | Low | 1.431 | 0.381 | 3.760 | <0.001 | Cluster | 32 |
| 35 | Alpha.Score | Cluster | Low | Other | −0.502 | 0.524 | −0.958 | ≥0.25 | Cluster | 35 |
Click on tabs to display tables. Scroll to see additional rows.
| ADONIS2 | ||||||
| adonis2(Beta Distance ~ Temperature*Cluster); Exposed fish | ||||||
| term | df | SumOfSqs | R2 | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 5.218 | 0.251 | 18.730 | 0.001 | *** |
| Cluster | 2.000 | 3.594 | 0.173 | 12.902 | 0.001 | *** |
| Temperature:Cluster | 3.000 | 0.653 | 0.031 | 1.564 | 0.061 | ns |
| Residual | 81.000 | 11.282 | 0.544 | NA | NA | NA |
| Total | 88.000 | 20.748 | 1.000 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 3.573 | 0.117 | 6.064 | 0.001 | *** |
| Cluster | 2.000 | 1.928 | 0.063 | 3.273 | 0.001 | *** |
| Temperature:Cluster | 3.000 | 1.157 | 0.038 | 1.310 | 0.057 | ns |
| Residual | 81.000 | 23.862 | 0.782 | NA | NA | NA |
| Total | 88.000 | 30.520 | 1.000 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 3.363 | 0.212 | 14.844 | 0.001 | *** |
| Cluster | 2.000 | 2.657 | 0.168 | 11.728 | 0.001 | *** |
| Temperature:Cluster | 3.000 | 0.645 | 0.041 | 1.898 | 0.025 | * |
| Residual | 81.000 | 9.176 | 0.579 | NA | NA | NA |
| Total | 88.000 | 15.841 | 1.000 | NA | NA | NA |
| ANOVA: Homogeneity of Dispersion | ||||||
| ANOVA(Beta Disperson ~ Temperature*Cluster); Exposed fish | ||||||
| term | df | sumsq | meansq | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Cluster | 2.000 | 0.019 | 0.009 | 0.383 | ≥0.25 | ns |
| Residual | 86.000 | 2.116 | 0.025 | NA | NA | NA |
| canberra | ||||||
| Cluster | 2.000 | 0.167 | 0.084 | 10.322 | <0.001 | **** |
| Residual | 86.000 | 0.697 | 0.008 | NA | NA | NA |
| gunifrac | ||||||
| Cluster | 2.000 | 0.016 | 0.008 | 0.628 | ≥0.25 | ns |
| Residual | 86.000 | 1.078 | 0.013 | NA | NA | NA |
| Tukey: Homogeneity of Dispersion | ||||||||
| Tukey(Beta Disperson ~ Temperature*Cluster); Exposed fish | ||||||||
| .y. | term | group1 | group2 | estimate | conf.low | conf.high | adj.p.value | sig |
|---|---|---|---|---|---|---|---|---|
| bray | ||||||||
| Distance | Cluster | Low | Other | −0.037 | −0.138 | 0.064 | ≥0.25 | ns |
| Distance | Cluster | High | Other | −0.005 | −0.114 | 0.104 | ≥0.25 | ns |
| Distance | Cluster | High | Low | 0.032 | −0.099 | 0.163 | ≥0.25 | ns |
| canberra | ||||||||
| Distance | Cluster | Low | Other | 0.098 | 0.040 | 0.156 | <0.001 | *** |
| Distance | Cluster | High | Other | −0.031 | −0.093 | 0.032 | ≥0.25 | ns |
| Distance | Cluster | High | Low | −0.129 | −0.204 | −0.053 | <0.001 | *** |
| gunifrac | ||||||||
| Distance | Cluster | Low | Other | 0.017 | −0.055 | 0.089 | ≥0.25 | ns |
| Distance | Cluster | High | Other | −0.027 | −0.104 | 0.051 | ≥0.25 | ns |
| Distance | Cluster | High | Low | −0.044 | −0.137 | 0.050 | ≥0.25 | ns |
Click on tabs to reveal supplementary figures and tables.
Click on tabs to cycle through different cluster comparisons between alpha and beta diversity.
Fig. 6 Water temperature influences gut microbiome response to Pseudocapillaria tomentosa. (A) Simpson’s Index for diversity of parasite unexposed and pre-exposed fish at 0 days post exposure (dpe). Prior to parasite exposure gut microbial alpha-diversity does not significantly differ between fish reared at the same water temperature. (B) Simpson’s Index for diversity of parasite unexposed and exposed fish. Gut microbial alpha-diversity significantly differs between parasite exposed fish reared at 28°C and 32°C water temperature relative to unexposed control fish, but gut microbial alpha-diversity does not differ between parasite unexposed and exposed fish reared at 35°C water temperature. Capscale ordinations based on the Bray-Curtis dissimilarity of gut microbiome composition constrained on the main and interaction effects of temperature and parasite exposure (treatment) (C) of pre-exposed samples at 0 dpe, and (D) post-exposed samples after 0 dpe. The analysis shows that gut microbiome composition of fish significantly differs between water temperature and parasite exposure in pre- and post-exposed fish. However, the interaction effect between temperature and parasite exposure is not significant in pre-exposed fish, but is significant in post-exposed fish. Ribbons and ellipses indicate 95% confidence interval. Ribbons and ellipses indicate 95% confidence interval. Only statistically significant relationships are shown. A “*” indicates statistical significance below the “0.05” level. Black arrows indicate statistically significant covariates and direction of greatest change in the indicated covariates.
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| GLM Results | |||||
| glm(Alpha.Score ~ Temperature*Treatment); Pre-Exposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| Shannon | |||||
| (Intercept) | −0.218 | 0.269 | −0.811 | ≥0.25 | ns |
| Temperature32 | 0.345 | 0.379 | 0.910 | ≥0.25 | ns |
| Temperature35 | 0.693 | 0.384 | 1.803 | 0.077 | ns |
| TreatmentExposed | 0.274 | 0.379 | 0.723 | ≥0.25 | ns |
| Temperature32:TreatmentExposed | 0.311 | 0.544 | 0.572 | ≥0.25 | ns |
| Temperature35:TreatmentExposed | 0.041 | 0.550 | 0.075 | ≥0.25 | ns |
| Simpson | |||||
| (Intercept) | −0.241 | 0.252 | −0.958 | ≥0.25 | ns |
| Temperature32 | 0.352 | 0.355 | 0.990 | ≥0.25 | ns |
| Temperature35 | 0.961 | 0.367 | 2.619 | 0.011 | * |
| TreatmentExposed | 0.223 | 0.355 | 0.627 | ≥0.25 | ns |
| Temperature32:TreatmentExposed | 0.486 | 0.513 | 0.948 | ≥0.25 | ns |
| Temperature35:TreatmentExposed | 0.017 | 0.525 | 0.033 | ≥0.25 | ns |
| Richness | |||||
| (Intercept) | −0.145 | 0.270 | −0.538 | ≥0.25 | ns |
| Temperature32 | 0.550 | 0.385 | 1.430 | 0.158 | ns |
| Temperature35 | 0.043 | 0.381 | 0.112 | ≥0.25 | ns |
| TreatmentExposed | 0.402 | 0.382 | 1.050 | ≥0.25 | ns |
| Temperature32:TreatmentExposed | 0.020 | 0.554 | 0.036 | ≥0.25 | ns |
| Temperature35:TreatmentExposed | 0.287 | 0.545 | 0.526 | ≥0.25 | ns |
| Phylogenetic | |||||
| (Intercept) | −0.253 | 0.254 | −0.996 | ≥0.25 | ns |
| Temperature32 | 0.553 | 0.360 | 1.536 | 0.130 | ns |
| Temperature35 | 0.171 | 0.358 | 0.478 | ≥0.25 | ns |
| TreatmentExposed | 0.335 | 0.358 | 0.937 | ≥0.25 | ns |
| Temperature32:TreatmentExposed | −0.018 | 0.512 | −0.035 | ≥0.25 | ns |
| Temperature35:TreatmentExposed | 0.102 | 0.507 | 0.201 | ≥0.25 | ns |
| ANOVA of GLM | ||||
| ANOVA(GLM(Alpha.Score ~ Temperature*Treatment), type = 2); Pre-Exposed fish | ||||
| term | statistic | df | p.value | sig |
|---|---|---|---|---|
| Shannon | ||||
| Temperature | 7.264 | 2.000 | 0.026 | * |
| Treatment | 3.044 | 1.000 | 0.081 | ns |
| Temperature:Treatment | 0.380 | 2.000 | ≥0.25 | ns |
| Simpson | ||||
| Temperature | 14.462 | 2.000 | <0.001 | *** |
| Treatment | 3.406 | 1.000 | 0.065 | ns |
| Temperature:Treatment | 1.122 | 2.000 | ≥0.25 | ns |
| Richness | ||||
| Temperature | 4.311 | 2.000 | 0.116 | ns |
| Treatment | 5.067 | 1.000 | 0.024 | * |
| Temperature:Treatment | 0.340 | 2.000 | ≥0.25 | ns |
| Phylogenetic | ||||
| Temperature | 4.600 | 2.000 | 0.100 | ns |
| Treatment | 3.056 | 1.000 | 0.080 | ns |
| Temperature:Treatment | 0.064 | 2.000 | ≥0.25 | ns |
| Pairwise Tukey's HSD, p.adj: Dunnett | ||||||||||
| Tukey(Alpha.Score ~ Temperature*Treatment); Pre-Exposed fish | ||||||||||
| Temperature | .y. | term | group1 | group2 | estimate | std.error | statistic | adj.p.value | Variable | Group |
|---|---|---|---|---|---|---|---|---|---|---|
| Shannon | ||||||||||
| 28 | Alpha.Score | Treatment | Exposed | Control | 0.274 | 0.364 | 0.753 | ≥0.25 | Treatment | 28 |
| 32 | Alpha.Score | Treatment | Exposed | Control | 0.585 | 0.451 | 1.298 | 0.194 | Treatment | 32 |
| 35 | Alpha.Score | Treatment | Exposed | Control | 0.315 | 0.344 | 0.917 | ≥0.25 | Treatment | 35 |
| Simpson | ||||||||||
| 28 | Alpha.Score | Treatment | Exposed | Control | 0.223 | 0.337 | 0.662 | ≥0.25 | Treatment | 28 |
| 32 | Alpha.Score | Treatment | Exposed | Control | 0.709 | 0.426 | 1.663 | 0.096 | Treatment | 32 |
| 35 | Alpha.Score | Treatment | Exposed | Control | 0.240 | 0.339 | 0.708 | ≥0.25 | Treatment | 35 |
| Richness | ||||||||||
| 28 | Alpha.Score | Treatment | Exposed | Control | 0.402 | 0.361 | 1.112 | ≥0.25 | Treatment | 28 |
| 32 | Alpha.Score | Treatment | Exposed | Control | 0.422 | 0.411 | 1.027 | ≥0.25 | Treatment | 32 |
| 35 | Alpha.Score | Treatment | Exposed | Control | 0.689 | 0.400 | 1.721 | 0.085 | Treatment | 35 |
| Phylogenetic | ||||||||||
| 28 | Alpha.Score | Treatment | Exposed | Control | 0.335 | 0.344 | 0.974 | ≥0.25 | Treatment | 28 |
| 32 | Alpha.Score | Treatment | Exposed | Control | 0.317 | 0.348 | 0.911 | ≥0.25 | Treatment | 32 |
| 35 | Alpha.Score | Treatment | Exposed | Control | 0.437 | 0.389 | 1.123 | ≥0.25 | Treatment | 35 |
Click on tabs to display tables. Scroll to see additional rows.
| GLM Results | |||||
| glm(Alpha.Score ~ Temperature*Treatment); Post-Exposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| Shannon | |||||
| (Intercept) | −0.441 | 0.126 | −3.499 | <0.001 | *** |
| Temperature32 | −0.064 | 0.177 | −0.364 | ≥0.25 | ns |
| Temperature35 | −0.087 | 0.182 | −0.476 | ≥0.25 | ns |
| TreatmentExposed | −0.539 | 0.210 | −2.562 | 0.011 | * |
| Temperature32:TreatmentExposed | 0.953 | 0.280 | 3.405 | <0.001 | *** |
| Temperature35:TreatmentExposed | 0.561 | 0.281 | 1.996 | 0.047 | * |
| Simpson | |||||
| (Intercept) | −0.299 | 0.133 | −2.252 | 0.025 | * |
| Temperature32 | −0.059 | 0.186 | −0.319 | ≥0.25 | ns |
| Temperature35 | 0.080 | 0.190 | 0.419 | ≥0.25 | ns |
| TreatmentExposed | −0.724 | 0.225 | −3.223 | 0.001 | ** |
| Temperature32:TreatmentExposed | 1.106 | 0.298 | 3.715 | <0.001 | *** |
| Temperature35:TreatmentExposed | 0.673 | 0.297 | 2.270 | 0.024 | * |
| Richness | |||||
| (Intercept) | −0.206 | 0.101 | −2.040 | 0.043 | * |
| Temperature32 | −0.048 | 0.141 | −0.343 | ≥0.25 | ns |
| Temperature35 | −0.317 | 0.147 | −2.164 | 0.032 | * |
| TreatmentExposed | −0.228 | 0.160 | −1.421 | 0.157 | ns |
| Temperature32:TreatmentExposed | 0.829 | 0.219 | 3.776 | <0.001 | *** |
| Temperature35:TreatmentExposed | 0.691 | 0.219 | 3.155 | 0.002 | ** |
| Phylogenetic | |||||
| (Intercept) | −0.143 | 0.095 | −1.507 | 0.134 | ns |
| Temperature32 | −0.035 | 0.132 | −0.268 | ≥0.25 | ns |
| Temperature35 | −0.319 | 0.138 | −2.320 | 0.021 | * |
| TreatmentExposed | −0.192 | 0.150 | −1.280 | 0.202 | ns |
| Temperature32:TreatmentExposed | 0.780 | 0.206 | 3.783 | <0.001 | *** |
| Temperature35:TreatmentExposed | 0.639 | 0.205 | 3.111 | 0.002 | ** |
| ANOVA of GLM | ||||
| ANOVA(GLM(Alpha.Score ~ Temperature*Treatment), type = 2); Post-Exposed fish | ||||
| term | statistic | df | p.value | sig |
|---|---|---|---|---|
| Shannon | ||||
| Temperature | 5.908 | 2.000 | 0.052 | ns |
| Treatment | 0.001 | 1.000 | ≥0.25 | ns |
| Temperature:Treatment | 11.784 | 2.000 | 0.003 | ** |
| Simpson | ||||
| Temperature | 8.400 | 2.000 | 0.015 | * |
| Treatment | 0.547 | 1.000 | ≥0.25 | ns |
| Temperature:Treatment | 14.128 | 2.000 | <0.001 | *** |
| Richness | ||||
| Temperature | 11.325 | 2.000 | 0.003 | ** |
| Treatment | 11.824 | 1.000 | <0.001 | *** |
| Temperature:Treatment | 16.178 | 2.000 | <0.001 | *** |
| Phylogenetic | ||||
| Temperature | 13.345 | 2.000 | 0.001 | ** |
| Treatment | 13.294 | 1.000 | <0.001 | *** |
| Temperature:Treatment | 16.080 | 2.000 | <0.001 | *** |
| Pairwise Tukey's HSD, p.adj: Dunnett | ||||||||||
| Tukey(Alpha.Score ~ Temperature*Treatment); Post-Exposed fish | ||||||||||
| Temperature | .y. | term | group1 | group2 | estimate | std.error | statistic | adj.p.value | Variable | Group |
|---|---|---|---|---|---|---|---|---|---|---|
| Shannon | ||||||||||
| 28 | Alpha.Score | Treatment | Exposed | Control | −0.539 | 0.280 | −1.924 | 0.054 | Treatment | 28 |
| 32 | Alpha.Score | Treatment | Exposed | Control | 0.414 | 0.180 | 2.298 | 0.022 | Treatment | 32 |
| 35 | Alpha.Score | Treatment | Exposed | Control | 0.022 | 0.111 | 0.200 | ≥0.25 | Treatment | 35 |
| Simpson | ||||||||||
| 28 | Alpha.Score | Treatment | Exposed | Control | −0.724 | 0.290 | −2.492 | 0.013 | Treatment | 28 |
| 32 | Alpha.Score | Treatment | Exposed | Control | 0.382 | 0.190 | 2.013 | 0.044 | Treatment | 32 |
| 35 | Alpha.Score | Treatment | Exposed | Control | −0.051 | 0.130 | −0.387 | ≥0.25 | Treatment | 35 |
| Richness | ||||||||||
| 28 | Alpha.Score | Treatment | Exposed | Control | −0.228 | 0.223 | −1.021 | ≥0.25 | Treatment | 28 |
| 32 | Alpha.Score | Treatment | Exposed | Control | 0.601 | 0.132 | 4.546 | <0.001 | Treatment | 32 |
| 35 | Alpha.Score | Treatment | Exposed | Control | 0.463 | 0.092 | 5.021 | <0.001 | Treatment | 35 |
| Phylogenetic | ||||||||||
| 28 | Alpha.Score | Treatment | Exposed | Control | −0.192 | 0.210 | −0.916 | ≥0.25 | Treatment | 28 |
| 32 | Alpha.Score | Treatment | Exposed | Control | 0.588 | 0.121 | 4.858 | <0.001 | Treatment | 32 |
| 35 | Alpha.Score | Treatment | Exposed | Control | 0.447 | 0.090 | 4.961 | <0.001 | Treatment | 35 |
Click on tabs to display tables. Scroll to see additional rows.
| ADONIS2 | ||||||
| adonis2(Beta Distance ~ Temperature*Treatment); Pre-Exposed fish | ||||||
| term | df | SumOfSqs | R2 | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 0.871 | 0.188 | 7.178 | 0.001 | *** |
| Treatment | 1.000 | 0.244 | 0.053 | 4.025 | 0.003 | ** |
| Temperature:Treatment | 2.000 | 0.245 | 0.053 | 2.019 | 0.046 | * |
| Residual | 54.000 | 3.275 | 0.707 | NA | NA | NA |
| Total | 59.000 | 4.635 | 1.000 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 1.652 | 0.132 | 4.615 | 0.001 | *** |
| Treatment | 1.000 | 0.533 | 0.043 | 2.979 | 0.001 | *** |
| Temperature:Treatment | 2.000 | 0.667 | 0.053 | 1.864 | 0.001 | *** |
| Residual | 54.000 | 9.665 | 0.772 | NA | NA | NA |
| Total | 59.000 | 12.517 | 1.000 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 0.647 | 0.153 | 5.604 | 0.001 | *** |
| Treatment | 1.000 | 0.288 | 0.068 | 4.987 | 0.002 | ** |
| Temperature:Treatment | 2.000 | 0.177 | 0.042 | 1.537 | 0.107 | ns |
| Residual | 54.000 | 3.117 | 0.737 | NA | NA | NA |
| Total | 59.000 | 4.229 | 1.000 | NA | NA | NA |
| ANOVA: Homogeneity of Dispersion | ||||||
| ANOVA(Beta Disperson ~ Temperature); Pre-Exposed fish | ||||||
| term | df | sumsq | meansq | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temp.Treat | 5.000 | 0.103 | 0.021 | 2.738 | 0.028 | * |
| Residual | 54.000 | 0.405 | 0.007 | NA | NA | NA |
| canberra | ||||||
| Temp.Treat | 5.000 | 0.014 | 0.003 | 0.453 | ≥0.25 | ns |
| Residual | 54.000 | 0.329 | 0.006 | NA | NA | NA |
| gunifrac | ||||||
| Temp.Treat | 5.000 | 0.054 | 0.011 | 1.564 | 0.186 | ns |
| Residual | 54.000 | 0.369 | 0.007 | NA | NA | NA |
| Tukey: Homogeneity of Dispersion | ||||||||
| Tukey(Beta Disperson ~ Temperature*Treatment); Pre-Exposed fish | ||||||||
| .y. | term | group1 | group2 | estimate | conf.low | conf.high | adj.p.value | sig |
|---|---|---|---|---|---|---|---|---|
| bray | ||||||||
| Distance | Temp.Treat | 28°C__Exposed | 28°C__Control | 0.027 | −0.088 | 0.141 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Control | 28°C__Control | 0.095 | −0.019 | 0.210 | 0.153 | ns |
| Distance | Temp.Treat | 32°C__Exposed | 28°C__Control | 0.110 | −0.004 | 0.225 | 0.065 | ns |
| Distance | Temp.Treat | 35°C__Control | 28°C__Control | 0.015 | −0.099 | 0.130 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 28°C__Control | 0.069 | −0.045 | 0.183 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Control | 28°C__Exposed | 0.069 | −0.046 | 0.183 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Exposed | 28°C__Exposed | 0.084 | −0.031 | 0.198 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Control | 28°C__Exposed | −0.011 | −0.126 | 0.103 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 28°C__Exposed | 0.042 | −0.072 | 0.157 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Exposed | 32°C__Control | 0.015 | −0.100 | 0.129 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Control | 32°C__Control | −0.080 | −0.195 | 0.034 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 32°C__Control | −0.026 | −0.141 | 0.088 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Control | 32°C__Exposed | −0.095 | −0.209 | 0.019 | 0.156 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 32°C__Exposed | −0.041 | −0.156 | 0.073 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 35°C__Control | 0.054 | −0.061 | 0.168 | ≥0.25 | ns |
| canberra | ||||||||
| Distance | Temp.Treat | 28°C__Exposed | 28°C__Control | 0.011 | −0.092 | 0.114 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Control | 28°C__Control | 0.035 | −0.068 | 0.138 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Exposed | 28°C__Control | 0.044 | −0.059 | 0.147 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Control | 28°C__Control | 0.010 | −0.094 | 0.113 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 28°C__Control | 0.016 | −0.087 | 0.119 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Control | 28°C__Exposed | 0.024 | −0.079 | 0.127 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Exposed | 28°C__Exposed | 0.033 | −0.070 | 0.136 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Control | 28°C__Exposed | −0.001 | −0.104 | 0.102 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 28°C__Exposed | 0.005 | −0.098 | 0.108 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Exposed | 32°C__Control | 0.009 | −0.094 | 0.112 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Control | 32°C__Control | −0.025 | −0.128 | 0.078 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 32°C__Control | −0.019 | −0.122 | 0.084 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Control | 32°C__Exposed | −0.034 | −0.137 | 0.069 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 32°C__Exposed | −0.028 | −0.131 | 0.075 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 35°C__Control | 0.006 | −0.097 | 0.109 | ≥0.25 | ns |
| gunifrac | ||||||||
| Distance | Temp.Treat | 28°C__Exposed | 28°C__Control | 0.003 | −0.106 | 0.112 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Control | 28°C__Control | 0.051 | −0.058 | 0.160 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Exposed | 28°C__Control | 0.078 | −0.032 | 0.187 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Control | 28°C__Control | 0.000 | −0.109 | 0.110 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 28°C__Control | 0.042 | −0.067 | 0.151 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Control | 28°C__Exposed | 0.048 | −0.061 | 0.158 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Exposed | 28°C__Exposed | 0.075 | −0.035 | 0.184 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Control | 28°C__Exposed | −0.002 | −0.112 | 0.107 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 28°C__Exposed | 0.039 | −0.070 | 0.148 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Exposed | 32°C__Control | 0.026 | −0.083 | 0.136 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Control | 32°C__Control | −0.051 | −0.160 | 0.059 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 32°C__Control | −0.009 | −0.118 | 0.100 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Control | 32°C__Exposed | −0.077 | −0.186 | 0.032 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 32°C__Exposed | −0.036 | −0.145 | 0.074 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 35°C__Control | 0.041 | −0.068 | 0.151 | ≥0.25 | ns |
Click on tabs to display tables. Scroll to see additional rows.
| ADONIS2 | ||||||
| adonis2(Beta Distance ~ Temperature*Treatment); Post-Exposed fish | ||||||
| term | df | SumOfSqs | R2 | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 4.123 | 0.108 | 17.035 | 0.001 | *** |
| Treatment | 1.000 | 7.411 | 0.194 | 61.235 | 0.001 | *** |
| Temperature:Treatment | 2.000 | 3.168 | 0.083 | 13.088 | 0.001 | *** |
| Residual | 194.000 | 23.478 | 0.615 | NA | NA | NA |
| Total | 199.000 | 38.179 | 1.000 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 4.561 | 0.074 | 9.502 | 0.001 | *** |
| Treatment | 1.000 | 7.402 | 0.120 | 30.840 | 0.001 | *** |
| Temperature:Treatment | 2.000 | 3.014 | 0.049 | 6.279 | 0.001 | *** |
| Residual | 194.000 | 46.561 | 0.757 | NA | NA | NA |
| Total | 199.000 | 61.538 | 1.000 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 2.273 | 0.082 | 11.945 | 0.001 | *** |
| Treatment | 1.000 | 4.718 | 0.170 | 49.577 | 0.001 | *** |
| Temperature:Treatment | 2.000 | 2.273 | 0.082 | 11.944 | 0.001 | *** |
| Residual | 194.000 | 18.460 | 0.666 | NA | NA | NA |
| Total | 199.000 | 27.724 | 1.000 | NA | NA | NA |
| ANOVA: Homogeneity of Dispersion | ||||||
| ANOVA(Beta Disperson ~ Temperature); Post-Exposed fish | ||||||
| term | df | sumsq | meansq | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temp.Treat | 5.000 | 1.344 | 0.269 | 17.882 | <0.001 | **** |
| Residual | 194.000 | 2.917 | 0.015 | NA | NA | NA |
| canberra | ||||||
| Temp.Treat | 5.000 | 1.094 | 0.219 | 31.758 | <0.001 | **** |
| Residual | 194.000 | 1.337 | 0.007 | NA | NA | NA |
| gunifrac | ||||||
| Temp.Treat | 5.000 | 1.225 | 0.245 | 29.264 | <0.001 | **** |
| Residual | 194.000 | 1.624 | 0.008 | NA | NA | NA |
| Tukey: Homogeneity of Dispersion | ||||||||
| Tukey(Beta Disperson ~ Temperature*Treatment); Post-Exposed fish | ||||||||
| .y. | term | group1 | group2 | estimate | conf.low | conf.high | adj.p.value | sig |
|---|---|---|---|---|---|---|---|---|
| bray | ||||||||
| Distance | Temp.Treat | 28°C__Exposed | 28°C__Control | 0.211 | 0.119 | 0.302 | <0.001 | **** |
| Distance | Temp.Treat | 32°C__Control | 28°C__Control | −0.015 | −0.096 | 0.066 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Exposed | 28°C__Control | 0.168 | 0.081 | 0.255 | <0.001 | **** |
| Distance | Temp.Treat | 35°C__Control | 28°C__Control | 0.013 | −0.070 | 0.096 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 28°C__Control | 0.060 | −0.024 | 0.144 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Control | 28°C__Exposed | −0.225 | −0.316 | −0.135 | <0.001 | **** |
| Distance | Temp.Treat | 32°C__Exposed | 28°C__Exposed | −0.043 | −0.138 | 0.053 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Control | 28°C__Exposed | −0.197 | −0.290 | −0.105 | <0.001 | **** |
| Distance | Temp.Treat | 35°C__Exposed | 28°C__Exposed | −0.150 | −0.243 | −0.057 | <0.001 | **** |
| Distance | Temp.Treat | 32°C__Exposed | 32°C__Control | 0.183 | 0.097 | 0.268 | <0.001 | **** |
| Distance | Temp.Treat | 35°C__Control | 32°C__Control | 0.028 | −0.054 | 0.110 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 32°C__Control | 0.075 | −0.008 | 0.158 | 0.100 | ns |
| Distance | Temp.Treat | 35°C__Control | 32°C__Exposed | −0.155 | −0.243 | −0.067 | <0.001 | **** |
| Distance | Temp.Treat | 35°C__Exposed | 32°C__Exposed | −0.108 | −0.196 | −0.019 | 0.007 | ** |
| Distance | Temp.Treat | 35°C__Exposed | 35°C__Control | 0.047 | −0.038 | 0.132 | ≥0.25 | ns |
| canberra | ||||||||
| Distance | Temp.Treat | 28°C__Exposed | 28°C__Control | 0.217 | 0.155 | 0.279 | <0.001 | **** |
| Distance | Temp.Treat | 32°C__Control | 28°C__Control | −0.011 | −0.066 | 0.044 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Exposed | 28°C__Control | 0.107 | 0.048 | 0.166 | <0.001 | **** |
| Distance | Temp.Treat | 35°C__Control | 28°C__Control | 0.013 | −0.044 | 0.069 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 28°C__Control | 0.073 | 0.016 | 0.130 | 0.004 | ** |
| Distance | Temp.Treat | 32°C__Control | 28°C__Exposed | −0.228 | −0.289 | −0.167 | <0.001 | **** |
| Distance | Temp.Treat | 32°C__Exposed | 28°C__Exposed | −0.110 | −0.175 | −0.045 | <0.001 | **** |
| Distance | Temp.Treat | 35°C__Control | 28°C__Exposed | −0.204 | −0.267 | −0.142 | <0.001 | **** |
| Distance | Temp.Treat | 35°C__Exposed | 28°C__Exposed | −0.144 | −0.207 | −0.081 | <0.001 | **** |
| Distance | Temp.Treat | 32°C__Exposed | 32°C__Control | 0.118 | 0.060 | 0.176 | <0.001 | **** |
| Distance | Temp.Treat | 35°C__Control | 32°C__Control | 0.024 | −0.032 | 0.080 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 32°C__Control | 0.084 | 0.028 | 0.140 | <0.001 | *** |
| Distance | Temp.Treat | 35°C__Control | 32°C__Exposed | −0.094 | −0.154 | −0.035 | <0.001 | *** |
| Distance | Temp.Treat | 35°C__Exposed | 32°C__Exposed | −0.034 | −0.094 | 0.026 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 35°C__Control | 0.060 | 0.003 | 0.118 | 0.035 | * |
| gunifrac | ||||||||
| Distance | Temp.Treat | 28°C__Exposed | 28°C__Control | 0.197 | 0.129 | 0.266 | <0.001 | **** |
| Distance | Temp.Treat | 32°C__Control | 28°C__Control | −0.023 | −0.083 | 0.037 | ≥0.25 | ns |
| Distance | Temp.Treat | 32°C__Exposed | 28°C__Control | 0.141 | 0.077 | 0.206 | <0.001 | **** |
| Distance | Temp.Treat | 35°C__Control | 28°C__Control | −0.011 | −0.073 | 0.051 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 28°C__Control | 0.054 | −0.009 | 0.117 | 0.134 | ns |
| Distance | Temp.Treat | 32°C__Control | 28°C__Exposed | −0.220 | −0.288 | −0.153 | <0.001 | **** |
| Distance | Temp.Treat | 32°C__Exposed | 28°C__Exposed | −0.056 | −0.128 | 0.015 | 0.211 | ns |
| Distance | Temp.Treat | 35°C__Control | 28°C__Exposed | −0.209 | −0.278 | −0.140 | <0.001 | **** |
| Distance | Temp.Treat | 35°C__Exposed | 28°C__Exposed | −0.143 | −0.213 | −0.074 | <0.001 | **** |
| Distance | Temp.Treat | 32°C__Exposed | 32°C__Control | 0.164 | 0.100 | 0.228 | <0.001 | **** |
| Distance | Temp.Treat | 35°C__Control | 32°C__Control | 0.012 | −0.050 | 0.073 | ≥0.25 | ns |
| Distance | Temp.Treat | 35°C__Exposed | 32°C__Control | 0.077 | 0.015 | 0.139 | 0.006 | ** |
| Distance | Temp.Treat | 35°C__Control | 32°C__Exposed | −0.152 | −0.218 | −0.087 | <0.001 | **** |
| Distance | Temp.Treat | 35°C__Exposed | 32°C__Exposed | −0.087 | −0.153 | −0.021 | 0.003 | ** |
| Distance | Temp.Treat | 35°C__Exposed | 35°C__Control | 0.065 | 0.002 | 0.129 | 0.039 | * |
Click on tabs to reveal supplementary figures and tables.
Fig. 7 A heatmap of model coefficient values of the top 50 statistically significant abundant gut microbial taxa identified by MaAsLin2. The color of each cell represents the coefficient value and direction (red is positive, blue is negative). A “+” or “-” indicates a statistically significant association was observed between taxon abundance and a covariate. Gray colored cells indicate a significant effect was not observed.
| Taxon | Temp: 32°C | Temp: 35°C | Time (DPE) | Cluster: Low | Parasite exposed | Infection present | Infection burden |
|---|---|---|---|---|---|---|---|
| Actinobacteriota | |||||||
| IMCC26207 | + | - | - | - | |||
| Nocardiaceae Genus | + | + | + | ||||
| PeM15 Genus | + | - | - | - | |||
| Bacteroidota | |||||||
| Barnesiellaceae Genus | - | - | |||||
| Cloacibacterium | + | - | |||||
| Flavobacterium | - | - | - | - | + | ||
| Fluviicola | - | - | - | - | |||
| Microscillaceae Genus | + | + | + | - | |||
| Terrimonas | - | + | + | ||||
| env OPS 17 Genus | - | - | - | ||||
| Chloroflexi | |||||||
| JG30 KF CM45 Genus | + | - | + | + | |||
| Cyanobacteria | |||||||
| Candidatus Obscuribacter | - | - | + | - | + | + | |
| Obscuribacteraceae Genus | + | + | - | + | - | ||
| Vampirovibrionaceae Genus | + | - | + | + | |||
| Desulfobacterota | |||||||
| Desulfobacterota Genus | - | + | + | ||||
| Firmicutes | |||||||
| Clostridium sensu stricto 3 | + | - | + | + | |||
| Gemmatimonadota | |||||||
| Gemmatimonadaceae Genus | - | - | + | + | |||
| Myxococcota | |||||||
| Sandaracinaceae Genus | + | + | + | - | + | + | |
| NB1-j | |||||||
| NB1 j Genus | + | + | - | + | + | ||
| Nitrospirota | |||||||
| Nitrospira | - | + | - | + | + | ||
| Patescibacteria | |||||||
| Saccharimonadales Genus | + | + | + | - | + | ||
| Planctomycetota | |||||||
| CL500 3 | + | - | - | + | |||
| Gemmata | + | + | - | + | + | ||
| Pirellulaceae Genus | + | - | + | - | - | ||
| Planctomycetales Genus | + | - | + | + | |||
| Planctomycetes Genus | + | - | + | + | |||
| Rhodopirellula | - | + | + | - | |||
| SM1A02 | + | - | + | + | |||
| Telmatocola | + | - | + | + | |||
| Proteobacteria | |||||||
| A0839 Genus | + | - | + | + | |||
| Alphaproteobacteria Genus | + | + | - | + | + | ||
| Bauldia | + | + | - | + | + | ||
| Beijerinckiaceae Genus | - | + | - | + | - | ||
| Bosea | - | - | - | - | |||
| Candidatus Alysiosphaera | + | + | - | + | + | ||
| Chitinibacter | + | + | + | - | |||
| Comamonadaceae Genus | - | - | + | + | |||
| Gammaproteobacteria Genus | - | - | + | - | + | ||
| Luteimonas | - | - | + | + | |||
| Methyloligellaceae Genus | + | + | + | - | + | + | |
| Nordella | - | - | + | + | |||
| Plesiomonas | + | + | - | - | - | ||
| Pseudomonadales Genus | - | - | - | - | - | - | |
| Rhizobacter | - | + | + | + | |||
| Rhizobiales Incertae Sedis Genus | - | + | |||||
| Rhodobacteraceae Genus | - | + | + | ||||
| Rhodovastum | - | - | |||||
| Steroidobacteraceae Genus | + | - | + | + | |||
| Xanthobacteraceae Genus | - | - | + | - | + | ||
| Verrucomicrobiota | |||||||
| Oikopleura | + | - | + | + | |||
Click on tabs to reveal supplementary figures and tables.
| All taxa with significant associations | |||||||
| MaAsLin2(log(qval)*sign(coef)); All fish | |||||||
| Taxon | Temp: 32°C | Temp: 35°C | Time (DPE) | Cluster: Low | Parasite exposed | Infection present | Infection burden |
|---|---|---|---|---|---|---|---|
| Acidobacteriota | |||||||
| Bryobacter | + | ||||||
| Holophagaceae Genus | - | - | + | ||||
| JGI 0001001 H03 | - | + | + | ||||
| Paludibaculum Genus | + | - | + | ||||
| Subgroup 17 Genus | - | + | |||||
| Vicinamibacteraceae Genus | - | + | + | ||||
| Actinobacteriota | |||||||
| Acidimicrobiia Genus | + | - | |||||
| Agromyces | + | + | |||||
| Brevibacterium | + | ||||||
| Conexibacter | + | + | + | ||||
| Corynebacteriales Genus | - | - | + | - | |||
| Frankiales Genus | + | - | + | + | |||
| Gaiella | - | + | |||||
| Gaiellales Genus | + | + | - | ||||
| Glutamicibacter | - | ||||||
| Gordonia | + | + | + | - | |||
| IMCC26207 | + | - | - | - | |||
| IMCC26256 Genus | + | + | - | + | |||
| Iamia | - | + | - | ||||
| Ilumatobacteraceae Genus | + | + | |||||
| Kineosporiaceae Genus | + | ||||||
| Leucobacter | - | ||||||
| Longivirga | + | - | |||||
| Microtrichaceae Genus | + | ||||||
| Microtrichales Genus | + | + | - | - | |||
| Mycobacterium | - | + | - | - | + | ||
| Nakamurella | - | ||||||
| Nocardia | - | + | |||||
| Nocardiaceae Genus | + | + | + | ||||
| Nocardioides | + | - | |||||
| PeM15 Genus | + | - | - | - | |||
| Rhodococcus | + | + | + | - | |||
| Smaragdicoccus | + | + | + | ||||
| Thermoleophilia Genus | - | + | + | ||||
| Armatimonadota | |||||||
| Armatimonadota Genus | + | ||||||
| Bacteroidota | |||||||
| AKYH767 Genus | - | - | + | ||||
| Aurantisolimonas | - | - | |||||
| Bacteroidia Genus | - | - | - | + | |||
| Barnesiellaceae Genus | - | - | |||||
| Candidatus Amoebophilus | + | ||||||
| Chitinophagaceae Genus | - | - | + | + | |||
| Chitinophagales Genus | - | - | + | ||||
| Chryseobacterium | + | ||||||
| Cloacibacterium | + | - | |||||
| Cytophaga | - | + | |||||
| Cytophagales Genus | - | - | + | ||||
| Dinghuibacter | + | + | - | - | |||
| Ferruginibacter | - | - | - | + | + | ||
| Flavihumibacter | - | - | - | - | |||
| Flavobacterium | - | - | - | - | + | ||
| Fluviicola | - | - | - | - | |||
| Haliscomenobacter | - | - | - | + | |||
| Hassallia | - | + | |||||
| Kapabacteriales Genus | - | - | + | ||||
| Microscillaceae Genus | + | + | + | - | |||
| NS9 marine group Genus | + | - | + | ||||
| OLB12 | + | + | - | + | |||
| Ohtaekwangia | + | + | + | ||||
| Runella | - | - | + | ||||
| Saprospiraceae Genus | - | - | + | ||||
| Sediminibacterium | - | ||||||
| Sphingobacteriales Genus | - | + | |||||
| Terrimonas | - | + | + | ||||
| env OPS 17 Genus | - | - | - | ||||
| Bdellovibrionota | |||||||
| Bacteriovoracaceae Genus | - | - | + | ||||
| Bdellovibrio | - | - | + | ||||
| OM27 clade | - | + | + | ||||
| Oligoflexaceae Genus | - | ||||||
| Chloroflexi | |||||||
| Caldilineaceae Genus | - | + | + | ||||
| JG30 KF CM45 Genus | + | - | + | + | |||
| KD4 96 Genus | + | - | + | ||||
| RBG 13 54 9 Genus | - | - | + | + | |||
| SBR1031 Genus | - | ||||||
| Cyanobacteria | |||||||
| Candidatus Obscuribacter | - | - | + | - | + | + | |
| Obscuribacteraceae Genus | + | + | - | + | - | ||
| Vampirovibrio | + | - | + | + | |||
| Vampirovibrionaceae Genus | + | - | + | + | |||
| Deinococcota | |||||||
| Deinococcus | - | ||||||
| Meiothermus | - | ||||||
| Dependentiae | |||||||
| Babeliaceae Genus | + | - | + | + | |||
| Desulfobacterota | |||||||
| Desulfobacterota Genus | - | + | + | ||||
| Desulfobulbus | - | + | |||||
| Elusimicrobiota | |||||||
| Lineage IIb Genus | - | ||||||
| Euryarchaeota | |||||||
| Methanobacteriaceae Genus | - | + | |||||
| Fibrobacterota | |||||||
| Fibrobacteraceae Genus | - | + | |||||
| Firmicutes | |||||||
| Acidaminobacter | - | + | + | ||||
| Anaerospora | + | ||||||
| Bacillus | + | - | - | ||||
| Brevibacillus | + | ||||||
| Clostridium sensu stricto 12 | - | + | |||||
| Clostridium sensu stricto 3 | + | - | + | + | |||
| Enterococcus | + | ||||||
| Geothermomicrobium | - | + | |||||
| Paraclostridium | + | + | + | - | |||
| Staphylococcus | + | ||||||
| Streptococcus | - | + | |||||
| Thermoactinomycetaceae Genus | + | + | |||||
| Vagococcus | + | ||||||
| ZOR0006 | - | - | - | ||||
| Fusobacteriota | |||||||
| Cetobacterium | + | + | - | - | - | ||
| Gemmatimonadota | |||||||
| Gemmatimonadaceae Genus | - | - | + | + | |||
| Halobacterota | |||||||
| Methanosarcina | + | ||||||
| MBNT15 | |||||||
| MBNT15 Genus | + | + | - | + | |||
| Myxococcota | |||||||
| Aetherobacter | + | - | + | ||||
| Anaeromyxobacter | - | + | |||||
| Blfdi19 Genus | - | ||||||
| Haliangium | - | - | + | + | |||
| P3OB 42 | - | + | |||||
| Pajaroellobacter | + | + | - | ||||
| Phaselicystis | - | + | |||||
| Polyangia Genus | - | ||||||
| Sandaracinaceae Genus | + | + | + | - | + | + | |
| mle1 27 Genus | - | + | |||||
| NB1-j | |||||||
| NB1 j Genus | + | + | - | + | + | ||
| Nitrospirota | |||||||
| Nitrospira | - | + | - | + | + | ||
| Patescibacteria | |||||||
| Saccharimonadales Genus | + | + | + | - | + | ||
| TM7a | + | ||||||
| Planctomycetota | |||||||
| BD7 11 Genus | - | + | |||||
| Blastopirellula | + | - | + | + | |||
| CL500 3 | + | - | - | + | |||
| Fimbriiglobus | + | - | + | - | + | + | |
| Gemmata | + | + | - | + | + | ||
| Gemmataceae Genus | - | - | + | ||||
| Isosphaeraceae Genus | + | - | + | + | + | ||
| OM190 Genus | - | + | + | + | |||
| Pir4 lineage | - | + | - | + | + | ||
| Pirellula | + | + | + | - | - | ||
| Pirellulaceae Genus | + | - | + | - | - | ||
| Planctomycetales Genus | + | - | + | + | |||
| Planctomycetes Genus | + | - | + | + | |||
| Planctomycetota Genus | - | + | |||||
| Rhodopirellula | - | + | + | - | |||
| SH PL14 | + | + | - | ||||
| SM1A02 | + | - | + | + | |||
| Schlesneria | - | - | + | + | |||
| Telmatocola | + | - | + | + | |||
| Tundrisphaera | + | - | - | - | - | ||
| vadinHA49 Genus | - | + | |||||
| Proteobacteria | |||||||
| A0839 Genus | + | - | + | + | |||
| AAP99 | - | + | |||||
| Acinetobacter | - | ||||||
| Aeromonas | - | + | + | ||||
| Ahniella | - | + | + | ||||
| Allorhizobium Neorhizobium Pararhizobium Rhizobium | - | ||||||
| Alphaproteobacteria Genus | + | + | - | + | + | ||
| Alsobacter | + | + | - | + | + | ||
| Aminobacter | - | - | |||||
| Aquabacterium | - | ||||||
| Aquicella | - | + | |||||
| Azospira | - | + | + | ||||
| Bauldia | + | + | - | + | + | ||
| Beijerinckiaceae Genus | - | + | - | + | - | ||
| Blastomonas | - | ||||||
| Bosea | - | - | - | - | |||
| Burkholderia Caballeronia Paraburkholderia | + | ||||||
| Burkholderiales Genus | - | ||||||
| CCM19a Genus | + | - | + | + | |||
| Caedibacter | + | - | + | ||||
| Candidatus Accumulibacter | - | + | - | ||||
| Candidatus Alysiosphaera | + | + | - | + | + | ||
| Candidatus Berkiella | - | + | - | ||||
| Candidatus Competibacter | - | ||||||
| Candidatus Megaira | + | - | + | ||||
| Candidatus Ovatusbacter | - | - | |||||
| Caulobacter | - | ||||||
| Chitinibacter | + | + | + | - | |||
| Chitinilyticum | - | - | |||||
| Chitinivorax | - | + | |||||
| Comamonadaceae Genus | - | - | + | + | |||
| Coxiella | - | + | |||||
| Crenobacter | - | ||||||
| Curvibacter | - | - | - | + | |||
| Dechlorobacter | - | + | |||||
| Dechloromonas | - | + | |||||
| Defluviimonas | - | - | - | ||||
| Diplorickettsiaceae Genus | - | ||||||
| Elstera | - | + | |||||
| Fodinicurvataceae Genus | - | + | |||||
| Ga0077536 Genus | - | - | + | ||||
| Gammaproteobacteria Genus | - | - | + | - | + | ||
| Gemmobacter | + | + | - | - | |||
| Hirschia | - | + | |||||
| Holosporaceae Genus | - | - | + | ||||
| Hydrogenophaga | + | ||||||
| Hyphomicrobium | + | + | |||||
| Ideonella | - | + | + | ||||
| Inhella | - | - | - | + | + | ||
| Leeia | - | - | - | + | |||
| Legionella | - | - | - | ||||
| Luteimonas | - | - | + | + | |||
| Magnetospirillum | - | + | |||||
| Mesorhizobium | - | ||||||
| Methyloligellaceae Genus | + | + | + | - | + | + | |
| Mycoplana | - | - | - | ||||
| Neisseriaceae Genus | - | ||||||
| Nitrosomonadaceae Genus | - | + | |||||
| Nitrosomonas | - | - | + | ||||
| Nordella | - | - | + | + | |||
| Novosphingobium | - | - | - | ||||
| Paucibacter | + | + | - | - | - | ||
| Pedomicrobium | + | + | + | - | |||
| Phenylobacterium | - | + | |||||
| Phreatobacter | - | - | |||||
| Plesiomonas | + | + | - | - | - | ||
| Polymorphobacter | - | - | + | + | |||
| Porticoccus | - | + | |||||
| Pseudomonadales Genus | - | - | - | - | - | - | |
| Pseudomonas | + | + | |||||
| Pseudorhodobacter | - | - | + | + | |||
| Pseudorhodoplanes | + | + | - | - | |||
| Reyranella | - | + | - | + | + | ||
| Rheinheimera | - | + | |||||
| Rhizobacter | - | + | + | + | |||
| Rhizobiaceae Genus | - | - | - | ||||
| Rhizobiales Incertae Sedis Genus | - | + | |||||
| Rhizorhapis | - | - | + | ||||
| Rhodobacteraceae Genus | - | + | + | ||||
| Rhodospirillales Genus | - | + | |||||
| Rhodovarius | - | + | + | ||||
| Rhodovastum | - | - | |||||
| Rickettsiaceae Genus | + | + | + | ||||
| Roseomonas | + | + | + | - | - | + | |
| Rubrivivax | - | - | |||||
| SC I 84 Genus | - | - | + | + | |||
| SWB02 | - | + | |||||
| Shewanella | - | - | - | ||||
| Shinella | - | - | - | ||||
| Sphingopyxis | + | ||||||
| Sphingorhabdus | - | - | + | + | |||
| Steroidobacter | + | - | |||||
| Steroidobacteraceae Genus | + | - | + | + | |||
| Sulfurifustis | + | ||||||
| Sulfuritalea | - | + | |||||
| Sutterellaceae Genus | + | - | - | - | |||
| TRA3 20 Genus | + | ||||||
| Tabrizicola | - | - | - | + | |||
| Undibacterium | - | - | - | + | |||
| Vibrio | - | - | + | + | - | ||
| Xanthobacteraceae Genus | - | - | + | - | + | ||
| Zoogloea | - | + | - | ||||
| alphaI cluster | + | - | - | ||||
| Spirochaetota | |||||||
| Turneriella | - | - | - | + | |||
| Verrucomicrobiota | |||||||
| Candidatus Protochlamydia | - | + | + | ||||
| Chlamydiales Genus | + | - | + | - | |||
| Chthoniobacter | - | - | - | + | - | ||
| DEV008 | - | + | |||||
| Luteolibacter | - | + | |||||
| Neochlamydia | + | - | - | ||||
| Oikopleura | + | - | + | + | |||
| Opitutaceae Genus | - | + | |||||
| Parachlamydiaceae Genus | - | ||||||
| Pedosphaeraceae Genus | - | - | + | + | |||
| SH3 11 | - | - | - | ||||
| Simkania | - | ||||||
| Simkaniaceae Genus | + | ||||||
| Verrucomicrobiaceae Genus | - | - | - | + | |||
| Verrucomicrobiae Genus | - | ||||||
| WPS-2 | |||||||
| WPS 2 Genus | - | + | |||||
| Summary of Significant Associations | |||||||
| (per covariate) | |||||||
| Significance | Cluster (Low) | DPE (DPE) | Pathology.Results (positive) | Temperature (32) | Temperature (35) | Total.Worm.Count (Total.Worm.Count) | Treatment (Exposed) |
|---|---|---|---|---|---|---|---|
| Positive | 1 | 73 | 117 | 42 | 37 | 49 | 74 |
| Negative | 192 | 36 | 5 | 47 | 54 | 13 | 35 |
| Summary of Significant Associations | |
| (per taxonomic level) | |
| Taxonomy | Count |
|---|---|
| Kingdom | 3 |
| Phylum | 28 |
| Class | 48 |
| Order | 114 |
| Family | 183 |
| Genus | 277 |